应用大鼠实验性痛经模型评价痛经停贴膏剂的镇痛作用  

Evaluation on Analgesic Effect of Tongjingting Paste in Rats Model with Experimental Dysmenorrhea

作　　者：刘瑾[1] 隋在云[1] 侯文静[1] 

机构地区：[1]山东省中医药研究院,济南250014

出　　处：《实验动物与比较医学》2015年第2期167-169,共3页Laboratory Animal and Comparative Medicine

基　　金：山东省中医药科技发展计划项目（No.2011-165）

摘　　要：目的应用大鼠实验性痛经模型评价痛经停贴膏剂的镇痛作用，初步探讨其治疗痛经的．作用机制。方法制备大鼠痛经模型，扭体法观察不同剂量痛经停贴膏剂对受试动物的镇痛作用，ELISA法测定痛经停贴膏剂对模型动物血浆6-酮-前列腺素1α（6-Keto-PGF1α）含量的影响。结果痛经停贴膏剂对实验性痛经大鼠具有明显镇痛作用，可明显减少受试动物的扭体次数，延长潜伏期，可使痛经大鼠血浆6-keto-PGF1α含量明显升高。结论痛经停贴膏剂对催产素所致子宫剧烈收缩的痛经大鼠有显著的镇痛作用，其镇痛机制与其对血浆6-keto-PGF1α水平的调节有关。Objective To evaluate the analgesic effect of tongjingting paste in rats model with experimental dysmenorrhea and to reveal its analgesic mechanism. Methods The experimental dys- menorrheal model in rats was induced with oxytocin. A writhing test was employed to evaluate the analgesic effects of tongjingting paste, which was designed into different doses, in animal subjects. The contents of 6-keto-PGF1α in the blood plasma were observed by ELISA assay. Results Tongjingting paste has a significant analgesic effect on experimental dysmenorrheal model in rats. Latent period was delayed significantly, the number of writhing response was evidently decreased as well. Besides, 6-keto- PGF1 A were significantly increased in the treated groups. Conclusion Tongjingting paste had a significant analgesic effect on experimental dysmenorrheal model in rats. Its analgesic mechanism may be mediated by regulating the plasma levels of 6-keto-PGF1α .

关 键 词：实验性痛经 痛经停贴膏剂 扭体实验 6-酮-前列腺素1α(6-Keto—PGF1α) 大鼠 

分 类 号：R285.5[医药卫生—中药学]