近红外发光葡聚糖纳米胶活体成像小鼠淋巴结  

Mapping of mouse lymph node with near-infrared emitting dextran nanogel in vivo

作　　者：李洁静[1] 蒋蓓绮 宋焱艳[2] 林超[2] 庄志刚[1] 

机构地区：[1]同济大学附属第一妇婴保健院乳腺外科,上海200092 [2]同济大学纳米研究院,上海200082

出　　处：《复旦学报（医学版）》2015年第2期230-234,249,共6页Fudan University Journal of Medical Sciences

基　　金：上海市科委医学引导类项目(124119a4700);上海市卫生局资助项目(20124164)~~

摘　　要：目的 制备和研究荧光染料标记的葡聚糖纳米胶,用于活体荧光成像小鼠腋窝淋巴结。方法 使用脱氧胆酸和荧光染料Cy7化学标记葡聚糖,通过透析法制备纳米胶Dextran-Cy7;使用透射电子显微镜、动态光散射仪分别表征纳米胶的形貌及大小;分光光度法标定Cy7的含量;用C57小鼠和DC2.4细胞分别评价DextranCy7的体内、体外毒性;对10只正常昆明小白鼠经前掌注射Dextran-Cy7生理盐溶液,使用活体荧光扫描成像系统观察小鼠腋窝淋巴结的成像效果;淋巴结免疫荧光组化法观察Dextran-Cy7在淋巴结内的分布。结果Dextran-Cy7纳米胶是直径30~50nm的球形纳米颗粒,其在体内和体外都具有较低的细胞毒性,可作为近红外发光探针活体荧光成像小鼠腋窝淋巴结;纳米胶主要分布在淋巴结的巨噬细胞细胞内。结论 Dextran-Cy7纳米胶细胞毒性低、生物相容性好,可作为纳米荧光探针成像活体小鼠淋巴结。Objective To prepare and investigate a near infrared-emitting nanogel for fluorescence mapping of mouse lymph node. Methods Dextran-Cy7 nanogel was prepared by the dialysis of deoxycholic acid and Cy7-conjugated dextran in water.The morphology and particle size of the nanogel were characterized by transmit electron microscope and dynamic light scattering meter,respectively.The Cy7 content in the nanogel was determined by UV-spectrophotometer analysis.In vitro and in vivo toxicity of Dextran-Cy7 was assessed using DC2.4cell line and C57 mouse mode,respectively.Ten Kunming mice were subcutaneously injected by the forepaw with Dextran-Cy7 and near-infrared fluorescence imaging of axillary lymph node was observed.The distribution of Dextran-Cy7 in the lymph node was detected by immunofluorescence staining. Results Dextran-CY7 nanogel showed spherical morphology with average particle size of about 30-50 nm.The nanogel has good cell compatibility in vivo and in vitro.Dextran-CY7 nanogel may be applied as a probe for near-infrared fluorescence mapping of mouse axillary lymph node.The nanogel was found mainly in the macrophages of the lymph node. Conclusions Dextran-Cy7 nanogel is low-toxic,biocompatible and may serve as a fluorescence tracer for in vivo fluorescence imaging of lymph node.

关 键 词：淋巴结 近红外荧光成像 荧光染料Cy7 葡聚糖 纳米胶 

分 类 号：R445[医药卫生—影像医学与核医学]