红掌‘Arizona’佛焰苞颜色部分突变的原因初探  

Preliminary Studies on Partial Mutation in Color of Anthurium andraeanum ‘Arizona' Spathe

作　　者：李崇晖[1] 牛俊海[1] 黄素荣[1] 王存[1] 陈金花[1] 杨光穗[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创制重点实验室,儋州571737

出　　处：《分子植物育种》2015年第3期627-633,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31101578);海南省重大科技专项项目(ZDZX2013012)共同资助

摘　　要：本研究以红掌红色品种‘Arizona’的一个有一半面积发生颜色由深红色突变为橙红色的佛焰苞为材料,将突变部位与正常部位对比,利用英国皇家园艺学会比色卡(RHSCC)和分光色差计比较了颜色差异,采用高效液相色谱(HPLC)分析了类黄酮色素的组成和含量变化,并通过实时荧光定量PCR分析了花青素合成途径关键酶基因的相对表达量变化。结果显示:与正常部位相比,突变部位的颜色变浅,彩度提高,偏向橙-红色;花青素苷组成由3个变为2个,以天竺葵素3-芸香糖苷为主(相对含量96.31%),未检测到芍药花素3-芸香糖苷,总花青素苷含量明显降低;黄酮苷和黄酮醇苷的组成未见变化,但总含量有所下降;关键酶基因除CHS略有上调外,F3H、DFR、ANS和F3'H表达量均发生不同程度的下调,其中ANS表达下调幅度最大,不到正常部位的1/7。研究结果表明‘Arizona’佛焰苞颜色突变是由于花青素合成关键酶基因表达量下调,使花青素苷组成发生变化,总含量降低,进而引起颜色变化。研究结果可为阐明红掌佛焰苞颜色调控的分子机制以及利用基因工程技术改良红掌佛焰苞颜色提供依据。The spathe with a half mutation color of dark red-colored Anthurium andraeanum cultivar‘Arizona＇was used as the material in this study. The mutant part was compared to the normal spathe color, which was performed by following the Royal Horticultural Society Colour Chart（RHSCC） as well as using a spectrophotometer. The composition and content of flavonoids were analyzed by high performance liquid chromatography（HPLC）. And the expression level of key enzyme genes involved in anthocyanin biosynthesis were detected through real-time PCR analysis. The results showed that the mutant spathe color turned to orange-red and appeared brighter and more vivid compared with the normal part. There were only two anthocyanins in the mutant part instead of three in the normal one. Pelargonidin 3-rutinoside was the absolutely major anthocyanin in the mutant part（with the percentage of 96.31%） whereas peonidin 3-rutinoside was not detected at all. The content of total anthocyanins reduced dramatically in the mutant part. The composition of flavone and flavonol glycosides was the same in the two parts,although the total contents of which decreased in the mutant part. When it came to key enzyme genes, besides CHS with a little up-regulation, the expression of F3 H, DFR, ANS and F3＇H were all down-regulated, among which the varied extant of ANS was the largest with less than a seventh relative expression amount of that in the normal part.The study suggested that the mutant color be caused by down-regulation of gene expression in anthocyanin biosynthesis, which might promote the changes in anthocyanin composition and the total content. These results could provide new insight into the mechanism of color modulation and the basis for the genetic engineering of spathe color improvement in Anthurium andraeanum.

关 键 词：红掌 颜色突变 花青素苷 基因表达 

分 类 号：S682.14[农业科学—观赏园艺]