盐胁迫下转过表达HS转录因子苜蓿突变体的生理生化变化  

Physiological and Biochemical Changes in Transgenic Alfalfa Mutants with Overexpression of HS Transcription Factor under Salt Stress Conditions

作　　者：米伟[1] 杨静慧[1,2] 程龙 梁燕[1] 姚宗国 刘艳军[1,2] 

机构地区：[1]天津农学院园艺园林学院,天津300384 [2]天津市中日农村环境资源合作研究中心,天津300384 [3]天津滨海新区大港林业工作站,天津300270

出　　处：《分子植物育种》2015年第3期658-662,共5页Molecular Plant Breeding

基　　金：国家农业科技成果转化资金项目(2012GB2A100015);天津市科委重大科技专项(12ZCDZNC04800)共同资助

摘　　要：为了研究抗逆HS转录因子基因的功能,本研究采用叶片离体盐胁迫的处理方法,比较了转基因苜蓿突变体chs1、chs2植株的生理生化变化。结果显示:在盐胁迫下,与对照相比,野生型苜蓿可溶性糖含量增加了2.68%,转基因chs1突变体增加了6.37%,chs2增加了12.36%;野生型苜蓿脯氨酸含量增加了27.91%,chs1突变体增加了37.17%,chs2增加了53.30%;野生型苜蓿丙二醛含量增加了12.58%,chs1突变体增加了7.31%,chs2增加了6.49%;野生型苜蓿细胞质膜透性增加了185.96%,chs1突变体增加了124.76%,chs2增加了119.61%;野生型苜蓿SOD活性降低了4.05%,chs1降低了2.75%,chs2降低3.27%;野生型苜蓿POD活性降低了16.62%,chs1降低了3.54%,chs2降低了16.43%。综合分析认为,转基因chs突变体的耐盐性强于野生型植株。因此,HS转录因子基因可以用于其它作物的耐盐育种。In order to understand the function of HS transcription factor, the physiological changes of transgenic Alfalfa mutant, Chs1, Chs2 were compared with vitro analysis and under salt stress of leaves. The result shows that the soluble sugar of wild plants, Chs1 and Chs2 was increased by 2.68%, 6.37% and 12.36% separately and their prolife was respectively up 27.91%, 37.17% and 53.30% and MDA rose up 12.58%, 7.31% and 6.49% severally under salt stress compared with normal growth condition. The cell membrane permeability of wild plants, Chs1 and Chs2 was separately more 185.96%, 124.76% and 119.61% under salt stress than plants under normal growth condition. The activity of SOD was decreased by 4.05%, 2.75% and 3.27% respectively and POD was reduced by16.62%, 3.54% and 16.43% separately under salt stress compared normal condition. In short, the salt tolerance of both transgenic Chs mutants are stronger than wild type plants. Accordingly, HS transcription factor can be used for salt tolerance breeding of other crops.

关 键 词：苜蓿 转录因子 转基因 生理生化 耐盐性 

分 类 号：S541.9[农业科学—作物学]