Bioautography and its scope in the field of natural product chemistry  

作　　者：Saikat Dewanjee Moumita Gangopadhyay Niloy Bhattacharya Ritu Khanra Tarun K.Dua 

机构地区：[1]Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University [2]Biophysics Division, Saha Institute of Nuclear Physics

出　　处：《Journal of Pharmaceutical Analysis》2015年第2期75-84,共10页药物分析学报（英文版）

摘　　要：Medicinal plants, vegetables and fruits are the sources of huge number of bioactive lead/scaffolds with therapeutic and nutraceutical importance. Bioautography is a means of target-directed isolation of active molecules on chromatogram. Organic solvents employed in chromatographic separation process can be completely removed before biological detection because these solvents cause inactivation of enzymes and/or death of living organisms. They offer a rapid and easy identification of bioactive lead/scaffolds in complex matrices of plant extracts. Bioautography is a technique to isolate hit（s）/lead（s） by employing a suitable chromatographic process followed by a biological detection system. This review critically describes the methodologies to identify antimicrobial, antioxidant, enzyme inhibitor lead/scaffolds by employing bioautography. A significant number of examples have been incorporated to authenticate the methodologies.Medicinal plants, vegetables and fruits are the sources of huge number of bioactive lead/scaffolds with therapeutic and nutraceutical importance. Bioautography is a means of target-directed isolation of active molecules on chromatogram. Organic solvents employed in chromatographic separation process can be completely removed before biological detection because these solvents cause inactivation of enzymes and/or death of living organisms. They offer a rapid and easy identification of bioactive lead/scaffolds in complex matrices of plant extracts. Bioautography is a technique to isolate hit（s）/lead（s） by employing a suitable chromatographic process followed by a biological detection system. This review critically describes the methodologies to identify antimicrobial, antioxidant, enzyme inhibitor lead/scaffolds by employing bioautography. A significant number of examples have been incorporated to authenticate the methodologies.

关 键 词：Bioactivity Bioassay Bioautography Detection principle Thin layer chromatography 

分 类 号：O629[理学—有机化学]