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作 者:V.Vijaya Bhaskar Anil Middha Pratima Srivastava Sriram Rajagopal
机构地区:[1]DMPK Laboratory (Biology Division), GVK BIO [2]Department of Pharmacy, Jagadishprasad Jhabermal Tibrewala University
出 处:《Journal of Pharmaceutical Analysis》2015年第2期120-129,共10页药物分析学报(英文版)
摘 要:A rapid, sensitive and selective pseudo MRM(p MRM)-based method for the determination of solutol HS15(SHS15) in rat plasma was developed using liquid chromatography/tandem mass spectrometry(LC–MS/MS). The most abundant ions corresponding to SHS15 free polyethyleneglycol(PEG)oligomers at m/z 481, 525, 569, 613, 657, 701, 745, 789, 833, 877, 921 and 965 were selected for p MRM in electrospray mode of ionization. Purity of the lipophilic and hydrophilic components of SHS15 was estimated using evaporative light scattering detector(ELSD). Plasma concentrations of SHS15 were measured after oral administration at 2.50 g/kg dose and intravenous administration at 1.00 g/kg dose in male Sprague Dawley rats. SHS15 has poor oral bioavailability of 13.74% in rats. Differences in pharmacokinetics of oligomers were studied. A novel proposal was conveyed to the scientific community,where formulation excipient could be analyzed as a qualifier in the analysis of new chemical entities(NCEs) to address the spiky plasma concentration profiles.A rapid, sensitive and selective pseudo MRM(p MRM)-based method for the determination of solutol HS15(SHS15) in rat plasma was developed using liquid chromatography/tandem mass spectrometry(LC–MS/MS). The most abundant ions corresponding to SHS15 free polyethyleneglycol(PEG)oligomers at m/z 481, 525, 569, 613, 657, 701, 745, 789, 833, 877, 921 and 965 were selected for p MRM in electrospray mode of ionization. Purity of the lipophilic and hydrophilic components of SHS15 was estimated using evaporative light scattering detector(ELSD). Plasma concentrations of SHS15 were measured after oral administration at 2.50 g/kg dose and intravenous administration at 1.00 g/kg dose in male Sprague Dawley rats. SHS15 has poor oral bioavailability of 13.74% in rats. Differences in pharmacokinetics of oligomers were studied. A novel proposal was conveyed to the scientific community,where formulation excipient could be analyzed as a qualifier in the analysis of new chemical entities(NCEs) to address the spiky plasma concentration profiles.
关 键 词:SHS15 LC–MS/MS Spiky profiles Validation
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