单侧横突拉簧加压建立兔脊柱侧凸模型  

Establishment of a rabbit model of scoliosis induced by asymmetric load using springs

作　　者：李伦超 刘湘[1] 朱青安[1] 黄志平[1] 任海龙[1] 郑帅[1] 李广军[1] 杨德鸿[1] 王吉兴[1] 

机构地区：[1]南方医科大学南方医院脊柱骨科,广东广州510515

出　　处：《南方医科大学学报》2015年第4期594-597,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(S2011010003918)

摘　　要：目的利用单侧横突拉簧加压法建立兔脊柱侧凸模型。方法两月龄新西兰白兔10只,手术予L2、L5右侧横突挂载拉簧。术前、术后1、4及8周摄X线正侧位片,测量Cobb角及拉簧长度,术后8周摄片后,随机将10只新西兰白兔分为两组:取拉簧(Spring removal,SR)组和保留拉簧组(Spring keeping,SK)各5只,取出SR组兔子体内拉簧,于9周和12周摄X线正侧位片,测量Cobb角及后凸角。12周后取所有兔顶椎及其上线椎体行Micro-CT扫描,记录顶椎上下终板的骨体积分数(BV/TV)。用方差分析或非参数检验及Student t检验对实验结果进行统计学分析。结果至实验结束时,兔全部存活,Cobb角均大于10°。术后1、4、8周不同时间点Cobb角及后凸角都大于术前(P<0.05)。术后8周,SR组与SK组的Cobb角、后凸角以及拉簧长度均无明显差异(P>0.05),取出拉簧后至12周,SK组的Cobb角及后凸角都较SR组要大(P<0.05),期间SR组的Cobb角及后凸角都逐渐减小。Micro-CT结果示凹侧终板骨体积分数要大于凸侧。整个实验过程中,拉簧形变量(ΔL)均无统计学差异(P>0.05)。结论单侧横突拉簧加压模型是一种可重复率高、简单易操作且实验周期短的动物脊柱侧凸模型。Objective To establish rabbit model of scoliosis induced with stable asymmetric lumbar loads. Methods Scoliosis was induced in 10 two-month-old New Zealand rabbits using 316L stainless steel springs placed between the unilateral transverse processes of L2 and L5. Serial radiographs were documented before and at 1, 4, 8, 9 and 12 weeks after the operation. At weeks, the rabbits were randomly divided into SR group （n=5） with the spring removed and SK group （n=5） without spring removal. Results All the rabbits survived the experiment with Cobb angle all greater than 10°at the end of the experiment. Significant changes were found in the Cobb angles and kyphotic angles at 1, 4 and 8 weeks after the operation （P〈0.05）. At 8 weeks, the Cobb angle, the kyphotic angle and the length of the spring were similar between SR and SK groups （P〈0.05）, and in the 4 weeks following spring removal in SR group, the Cobb angle and the kyphosis decreased significantly compared with those in SK group （P〈0.05）. Micro-CT showed that the BV/TV of the concave side was greater than that of the convex side. The length of the spring did not show obvious changes during the experiment （P〈0.05）. Conclusion Asymmetric lumbar loading is a convenient, time-saving, and highly reproducible approach for establishing rabbit models of scoliosis.

关 键 词：脊柱侧凸 模型/新西兰兔 拉簧 不对称载荷 

分 类 号：R682.3[医药卫生—骨科学] R-332[医药卫生—外科学]