HPLC法测定益心酮片中四种黄酮类成分的含量  

Determination of four flavonoid ingredients in Yixintong tablets by HPLC

作　　者：于淼 原源 

机构地区：[1]解放军85医院药剂科,上海200052

出　　处：《东南国防医药》2015年第2期157-159,165,共4页Military Medical Journal of Southeast China

摘　　要：目的建立高效液相色谱法（HPLC）同时测定益心酮片中牡荆素鼠李糖苷、牡荆素葡萄糖苷、牡荆素、金丝桃苷四种成分的含量。方法采用SHISEIDO CAPCELL Pak C18 AQ 250 mm×4.6 mm色谱柱;流动相为四氢呋喃-乙酸-水-甲醇-乙腈（38∶4∶152∶3∶3）;流速为1.0 m L/min;柱温：25℃;检测波长：330 nm。结果牡荆素鼠李糖苷、牡荆素葡萄糖苷、牡荆素、金丝桃苷的线性范围分别为10.11-201.02μg/m L（r=0.9997）,7.92~158.42μg/m L（r=0.9996）,4.02~80.42μg/m L（r=0.9995）,6.28~125.61μg/m L（r=0.9995）,范围内线性关系良好、精密度、重现性、回收率均符合要求。结论该方法快速、准确、重现性好可用于本品的质量控制。Objective To establish the quantitative analysis method for simultaneous determination of Vitexin rhamnosides,Vitexin glucoside,Vitexin and Hyperoside in Yixintong tablets by HPLC.Methods HPLC was performed on SHISEIDO CAPCELL Pak C18 AQ（4.6 mm × 250 mm）,with the mobile phase of THF-acetic acid-water-methanol-acetonitrile（38∶ 4∶ 152∶ 3∶ 3） at a flow rate of 1.0 m L / min.The column temperature was set at 25 ℃,and the detection wavelength was 330 nm.Results The linear ranges of Vitexin rhamnoside,Vitexin glucoside,Vitexin,Hyperoside were 10.11-201.02 μg / m L（r = 09997）,7.92-158.42 μg / m L（r= 0.9996）,4.02-80.42 μg / m L（r = 0.9995）,6.28-125.61 μg / m L（r = 0.9995） respectively.The linear relationship,precision,reproducibility,recovery rates all meet the requirements.Conclusion The content of Vitexin rhamnosides is significantly higher than Hyperoside,and the study shows that Vitexin rhamnosides has effect on dilating blood vessels,protecting cardiac tissue,which is consistent with the effect of the Yixintong tablets.Therefore,it＇s not reasonable to choose Hyperoside as the only quality control indicator of the Yixintong tablets in the 2010 Chinese Pharmacopoeia.Vitexin rhamnoside and Vitexin glucoside are also important pharmacological active components in Hawthorn Leaves,and could be considered as quality control indicators of the Yixintong tablets.

关 键 词：高效液相色谱法 益心酮片 牡荆素鼠李糖苷 牡荆素葡萄糖苷 牡荆素 金丝桃苷 

分 类 号：R286[医药卫生—中药学]