银合欢组织培养研究初报  

Preliminary Report on In Vitro Culture of Leucaena leucocephla(Lamarck) de Wit

作　　者：苏慕乔 周爽 白昌军[2] 唐军[2] 吴繁花[1] 蔡秀清[1] 刘进平[1] 

机构地区：[1]海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南大学农学院,海南海口570228 [2]中国热带农业科学院热带作物品种资源研究所,农业部热带作物种质资源利用重点开放实验室,海南儋州571737

出　　处：《热带农业工程》2015年第1期28-32,共5页Tropical Agricultural Engineering

摘　　要：以银合欢(Leucaena leucocephla(Lamarck)de Wit)无菌实生苗子叶和胚轴切段为外植体,以MS为基本培养基,研究不同BA和NAA浓度组合对银合欢组织培养效果的影响。结果表明:愈伤组织诱导培养中,以子叶的培养反应性最好,其愈伤组织诱导率在各种组合中都达到100%,其中以附加0.1 mg/L NAA和2.5-7.0 mg/L BA效果最好;胚轴在添加0.1 mg/L NAA及3.0-4.5 mg/L BA的MS培养基上培养时愈伤组织诱导率较高。子叶愈伤组织在MS+3.0 mg/L BA+0.2 mg/L NAA的培养基中培养时芽分化率只有6.7%;胚轴愈伤组织则未能实现芽的分化。The effect of different combinations of BA and NAA concentrations in basal MS medium on tissue culture of Leucaena leucocephla was studied with the segments of cotyledons and hypocotyls as explants. The results indicated that the cotyledons had better response for in vitro culture. The frequency of callus induction for the cotyledons was 100% on MS with all different combinations of BA and NAA and the calli grew better on MS with 0.1 mg/L NAA and 2.5-7.0 mg/L BA; The media（ MS ＋ 0.1 mg/L NAA ＋ 3.0-4.5 mg/L BA） were better for the callus induction of hypocotyls. The cotyledon calli on MS＋ 3.0 mg/L BA＋ 0.2 mg/L NAA differentiated into shoots with a shoot differentiation percentage of 6.7%;The calli induced from hypocotyls didn＇t achieve shoot differentiation.

关 键 词：银合欢 无菌萌发 组织培养 愈伤组织 

分 类 号：Q813.12[生物学—生物工程]