牛支原体内蒙古分离株脂蛋白vspY基因的克隆和序列分析  

Cloning and Sequence Analysis of Lipoprotein vspY Gene of Mycoplasma bovis Inner Mongolia Isolates

作　　者：宫利娜 郝瑞峰 王业华[1] 张岩[1] 高航飞[1] 李琛[1] 海日汗[1] 李平安[1] 吴太平[3] 关平原[1] 

机构地区：[1]内蒙古农业大学兽医学院农业部动物疾病临床诊疗技术重点实验室,内蒙古呼和浩特010018 [2]内蒙古托克托县农产品质量安全检验检测站,内蒙古托克托县010200 [3]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018

出　　处：《畜牧与饲料科学》2015年第3期9-13,共5页Animal Husbandry and Feed Science

基　　金：内蒙古自然科学基金(2014MS0361)

摘　　要：旨在为进一步研究牛支原体内蒙古分离株（NM 2012）的vsp Y1、vsp Y2基因功能提供依据。根据已发表的牛支原体vsp Y1、vsp Y2基因序列设计引物,对NM 2012株的vsp Y1、vsp Y2基因进行克隆、测序,并与已发表的相关序列进行相似性比较;采用在线生物信息学分析工具对NM 2012株的vsp Y1、vsp Y2推导的氨基酸的相似性、保守结构域、跨膜结构、信号肽、脂蛋白、抗原表位进行预测。结果表明,牛支原体内蒙古分离株（NM 2012）的vsp Y1、vsp Y2基因大小分别为1 029 bp和804 bp,分别编码342和267个氨基酸组成的完整开放阅读框;vsp Y1与已发表的牛支原体vsp Y1基因相似性为99.4%~100%,其推导的氨基酸序列相似性为98.6%~99.7%,vsp Y2与已发表的牛支原体vsp Y2基因相似性为100%。根据生物信息学软件分析结果推测,vsp Y1蛋白是潜在的毒力因子。The aim of this study was to provide basis for the further understanding of the functions of vsp Y1 and vsp Y2 genes of Mycoplasma bovis Inner Mongolia isolates（NM2012）. Two sets of primers were designed based on the published vsp Y1 and vsp Y2 genes of Mycoplasma bovis and used to amplify the vsp Y1 and vsp Y2 genes of NM 2012 isolate. The obtained sequences were cloned and sequenced. The nucleic acid sequence of vsp Y1 and vsp Y2 genes of NM 2012 isolate were compared with that of the related published genes respectively to identify their similarity. The similarity with other published or deduced amino acid,conserved domain, trans-membrane structure, signal peptide, lipoprotein and epitope of the amino acid sequences deduced by vsp Y1 and vsp Y2 genes were predicted by using online bioinformatics tools, respectively. The results showed that the size of vsp Y1 and vspY2 genes of NM 2012 isolate were 1 029 and 804 bp, respectively, and both of them contained complete open reading frames（ORF）, encoding 342 and 267 amino acids, respectively. The similarity of nucleic acid sequence between vsp Y1 gene of NM 2012 isolate and others related genes ranged from 99.4% to 100%, and that of the deduced amino acids sequence ranged from 98.6% to 99.7%. The similarity of nucleic acid sequence between vsp Y2 gene of NM 2012 isolate and others related genes were 100%. Based on the bioinformatics analysis results obtained in this study, it could be deduced that vsp Y1 was a potential virulence factor.

关 键 词：牛支原体 VSP Y1基因 VSP Y2基因 分子克隆 序列分析 

分 类 号：S852.62[农业科学—基础兽医学] Q523.8[农业科学—兽医学]