电针督脉后海穴对血管性痴呆大鼠学习记忆能力及海马CA1区JNK蛋白表达的影响  

Effect of Electro-Acupuncture on Houhai Acupoint on Learning and Memory Ability and JNK Protein Expression in Hippocampal CA1 of Rats with Vascular Dementia

作　　者：陈采益[1] 张学君[1] 林栋[1] 陈可爱[1] 洪霖[1] 

机构地区：[1]福建中医药大学针灸学院,福建福州350122

出　　处：《山东中医药大学学报》2015年第2期169-171,共3页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81202764);福建省教育厅省属高校科研专项(编号:JK2012022);福建中医药大学校管课题(编号:X2012019)

摘　　要：目的 :观察电针后海穴对血管性痴呆大鼠学习记忆能力及海马CA1区c-Jun氨基末端激酶(JNK)蛋白表达的影响。方法:将24只SD大鼠随机分为假手术组、模型组、电针后海组、电针非穴组,每组6只,采用双侧颈总动脉永久性结扎(2-VO)方法制备血管性痴呆模型,电针后海穴3个疗程后,采用Morris水迷宫检测各组大鼠学习记忆能力,免疫组化检测海马CA1区JNK蛋白表达情况。结果:与假手术组比较,模型组大鼠平均逃避潜伏期明显延长、穿越平台次数减少、海马CA1区JNK蛋白表达增高(P<0.05);与模型组比较,电针后海组的平均逃避潜伏期缩短、穿越平台次数增多、海马CA1区JNK蛋白表达降低(P<0.05),而电针非穴组未见显著性差异。结论:电针后海穴可改善血管性痴呆大鼠的学习记忆能力,其机制可能与海马CA1区JNK蛋白表达降低有关。Objective：To observe the effect of electro-acupuncture（EA） on Houhai acupoint on learning and memory ability and JNK protein expression in hippocampal CA1 of rats with vascular dementia（VD）. Methods：24 rats were randomly divided into sham operation group,model group,EA on Houhai group and EA non-acupoint group. Each group had 6 rats. Rats of VD models were established by 2 vessels obstruction（2-VO）.Three courses of electro-acupuncture on Houhai acupoint was given for rats in the EA on Houhai group.Morris water maze was used to assess their learning and memory ability and immunohistochemistry was used to assess the change of JNK protein in hippocampal CA1. Results：Compared with the sham operation group,the average latent period of the model group significantly prolonged,the times of crossing platform decreased and JNK protein expression of hippocampal CA1 increased（P 〈0.05）. Compared with the model group,the average latent period of EA on Houhai group shortened,the times of crossing platform increased and JNK protein expression of hippocampal CA1 decreased（P 〈0.05）. But EA non-acupoint group didn ＇t show significant difference. Conclusion：EA on Houhai acupoint can improve learning and memory ability of vascular dementia rats,whose mechanism is probably related to decreasing JNK protein expression in hippocampal CA1.

关 键 词：血管性痴呆 后海穴 电针 C-JUN氨基末端激酶 大鼠 

分 类 号：R245.97[医药卫生—针灸推拿学]