一个水稻类感干尖线虫卷叶突变体的遗传分析与基因定位  

Genetic Analysis and Molecular Mapping of a Nematode Infestation Mimic Rolling Leaf Mutant in Rice

作　　者：陈蕾[1,2] 李小华[3] 叶胜海[1] 张小明[1] 翟荣荣[1] 金庆生[1] 

机构地区：[1]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021 [2]南京野生植物综合利用研究院,江苏南京210042 [3]杭州师范大学生命与环境科学学院,浙江杭州310036

出　　处：《核农学报》2015年第4期617-622,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31101428);核农学产业科技创新团队(2010R50033);浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地;浙江省农业科学院提升工程项目;浙江省9410计划(2012C12901-2)

摘　　要：筛选和鉴定水稻卷叶突变体是研究叶片形态建成机理的前提。本研究利用甲基磺酸乙酯对常规粳稻秀水09进行诱变,获得了一个稳定遗传的类感干尖线虫卷叶突变体（nematode infestation mimic rolling leaf mutant,nir）。与野生型相比,nir全生育期叶尖卷曲、枯萎,表型与干尖线虫侵染症状相似。此外,突变体植株矮小,叶片表面较光滑,有效分蘖数少。采用nir与秀水09杂交的F2群体进行遗传分析,结果表明nir的类感干尖线虫卷叶性状受一对隐性核基因控制。利用nir与籼稻93-11杂交获得的F2群体,采用分子标记技术将目的基因最终定位在水稻第二染色体2~88w和2~82w之间,遗传距离为1.1 c M,物理距离245 kb。研究结果表明,nir很可能是一个尚未报道的新基因,进一步克隆nir基因有可能揭示与现有报道的卷叶基因不同的卷叶调控机理,为筛选理想株型、培育高产水稻品种提供理论基础和供试材料。Screening and identification of rice rolling leaf mutant is a premise for leaf morphogenesis mechanism,which provides theoretical basis and test materials for the obligation of rice with ideal plant type and high yield. In this study,a nematode infestation mimic rolling leaf mutant（ nir） was obtained from EMS-treated mutations in a japonica rice variety ‘Xiushui 09＇. Compared with the wild-type,nir showed obvious rolled and withered leaves in the whole stages,which was similar to the phenotype of rice plants infested by nematode. Furthermore,nir showed other characters such as dwarf plants,smooth leaves and less effective tillers. F2 population of nir /‘Xiushui 09 ＇was developed for genetic analysis,and the results showed that the mutant trait was controlled by a single recessive nuclear gene. Using amther F2 population of nir /93- 11 for gene mapping. By using the F2 populations,the gene controling the nematode infestation mimic rolling leaf trait was mapped to a region on chromosome 2 between two In Del markers,2 - 88 w and 2 - 82 w,with genetic distance of 1. 1 c M and physical distance of 245 kb. The results suggested that NIR might be a new gene that had not been reported before,and further cloning of NIR gene could reveal different regulation mechanism of rolling leaf.

关 键 词：水稻 类感干尖线虫 卷叶 遗传分析 基因定位 

分 类 号：S435.111.48[农业科学—农业昆虫与害虫防治]