雨生红球藻诱变后生长速率和超微结构的变化  

Changes in Growth Rate and Ultrastructure of Haematococcus pluvialis After Mutagenesis

作　　者：沈国明[1] 牛健康[1] 陆开形[2] 尚宏芹[1] 

机构地区：[1]菏泽学院生命科学系/植物生物学重点实验室,山东菏泽274015 [2]宁波大学科学技术学院,浙江宁波315211

出　　处：《核农学报》2015年第4期623-628,共6页Journal of Nuclear Agricultural Sciences

基　　金：菏泽学院引进博士启动基金(XY13BS01);菏泽学院植物生物学重点实验室建设项目;宁波市自然科学基金项目(2811A610002)

摘　　要：为探讨亚硝基胍(N-methyl-N'-Nitro-N-Nitrosoguanidine,NTG)对雨生红球藻(Haematococcus pluvialis)生长速率及超微结构变化的影响,用不同浓度的NTG处理藻类,对藻类的生长速率、色素含量和超微结构的变化进行了研究。结果表明:即使低浓度(0.5 g·L-1)的NTG对雨生红球藻也是致死的,致死率与NTG浓度呈正相关。藻类经中等浓度(2.5 g·L-1)NTG诱变后,存活藻类的增长速率K值和虾青素含量分别达到0.381±0.0289和2.9±0.29 mg·L-1。低浓度NTG处理藻类其叶绿素含量略有上升,但高浓度(3.5 g·L-1)时叶绿素含量明显减少。低NTG浓度处理时藻类细胞结构变化不大,但高浓度处理时细胞膜、线粒体及叶绿体等均遭受不同程度的损伤。本研究可为深入理解NTG诱变藻类的生理生化机制提供借鉴。To investigate the influcences of N- methyl- N＇- Nitro- N- Nitrosoguanidine（ NTG） on growth rate and ultrastructure of H. pluvialis（ Haematococcus pluvialis）,the growth rate,pigments content and ultrastructure were investigated after treated by NTG at different concentrations. The results showed that even a low concentration（ 0. 5g·L^- 1） of NTG was lethal for H. pluvialis and the lethality rate was related positively to NTG concentration. After mutated by medium concentration（ 2. 5g·L^- 1） of NTG,the growth rate（ K value） and astaxanthin amount of survival algae reached up to 0. 381 ± 0. 0289 and 2. 9 ± 0. 29 mg·L^- 1,respectively. The chlorophyll content increased slightly under a low NTG concentration but reduced with a high concentration. With low concentration of NTG,there were no changes in cell structure,but some organelles,such as cell membrane,mitochondria or chloroplast were damaged after treatment with high concentration of NTG（ 3. 5 g L^- 1）. This study provided insights for further understanding of the physiological and biochemical mechanisms of algae mutation by NTG.

关 键 词：生长率 雨生红球藻 致死率 亚硝基胍 色素含量 超微结构 

分 类 号：Q949.2[生物学—植物学]