微小RNA-206在系统性红斑狼疮患者外周血单个核细胞中表达的变化  

The level of miR-206 in per ipheral blood mononuclear cells and their signicance in systemic lupus erythematosus patients

作　　者：徐庆雷[1] 朱宝林[1] 马小波[1] 张巍[1] 刘玲玲[1] 王胜军[2] 

机构地区：[1]江苏省沭阳县人民医院检验科,223600 [2]江苏大学附属人民医院检验科

出　　处：《中华风湿病学杂志》2015年第4期254-256,共3页Chinese Journal of Rheumatology

摘　　要：目的 检测SLE患者PBMCs微小RNA （miR）-206表达的水平,探讨其在SLE发病中的作用.方法 收集SLE患者及健康对照外周血标本,分离PBMCs.采用实时定量（qRT）-PCR方法检测PBMCs中miR-206及锌指样转录因子4（KLF4）、孤儿核受体γt（RORγt）mRNA相对表达量.采用t检验和Spearman相关分析.结果 SLE患者PBMCs miR-206表达的水平显著低于健康对照组（0.066±0.021和0.143±0.059,t=3.136,P＜0.01）,KLF4 mRNA、RORγt mRNA表达水平显著高于健康对照组（0.637±0.186和0.104±0.028,t=6.673,P＜0.01）、（0.575±0.263和0.065 ±0.014,t=7.386,P＜0.01）,SLE患者PBMCs miR-206表达水平与KLF4、RORγt相对表达量均呈负相关（r=-0.627,P＜0.01）、（r=-0.853,P＜0.01）.结论 SLE患者PBMCsmiR-206表达的水平下降,与疾病的发生发展关系密切.Objective To explore the role of miR-206 in peripheral blood mononuclear cells （PBMCs） of systemic lupus erythematosus （SLE） patients.Methods Human PBMCs were isolated by standard densitygradient centrifugation over Ficoll-Hypaque solution in SLE and healthy controls.Total RNAs were extracted from PBMCs which were stimulated by PMA and ionomycin,thenthe RNA was transcribed reversely into cDNA.The expression of miR-206 and Kruppel-like factor 4 （KLF4） and the orphan nuclear receptor RORγt mRNA was detected by real-time quantitative polymerase chain reaction （qRT-PCR） method.T test and Spearman＇s correlation test were used for statistical analysis.Results The expression of miR-206 in the PBMCs of SLE patients was significantly decreased compared with that of healthy controls （0.066±0.021 vs 0.143±0.059,t=3.136,P〈0.01）.The levels of KLF4 and RORγt mRNAin the PBMCs of SLE patients were increased significantly than those of healthy controls （0.637 ±0.186 vs 0.104 ± 0.028,t=6.673,P〈0.01）,（0.575±0.263 vs 0.065±0.014,t=7.386,P〈0.01）.Furthermore,there was a negative correlation between the expression of miR-206 and KLF4 or RORγt mRNA in SLE patients （r=-0.627,P〈 0.01）,（r=-0.853,P〈0.01）.Conclusion These results indicate that the augmented expression of KLF4 mRNA may be caused by the attenuated expression of miR-206,and the high level of KLF4 mRNA evokes the proportion of Thl7 cells in SLE patients.

关 键 词：红斑狼疮 系统性 微小RNAS 锌指样转录因子4 孤儿核受体γt 

分 类 号：R593.241[医药卫生—内科学]