巨噬细胞磁共振成像在大鼠胶原诱导性关节炎中的应用研究  

Magnetic resonance imaging of mcrophages for the diagnosis of rat collagen-induced arthritis： a preliminary study

作　　者：徐蔷薇[1] 王美美[1] 张芹[1] 

机构地区：[1]东南大学附属中大医院风湿免疫科,南京210009

出　　处：《中华风湿病学杂志》2015年第4期257-261,F0003,共6页Chinese Journal of Rheumatology

基　　金：基金项目：江苏省自然科学基金（BK2009276）

摘　　要：目的 应用胶原诱导性关节炎（CIA）大鼠关节滑膜巨噬细胞MRI技术,观察超微超顺磁性氧化铁粒子（USPIO）增强前后不同序列、不同时间点及药物干预后关节滑膜巨噬细胞MRI信号的改变.方法 将造模成功大鼠分为模型组、来氟米特组、模型对照组.模型组大鼠行USPIO增强前及增强后24、48、72 h MRI扫描;来氟米特组大鼠每日给药8 mg/kg,14 d后行USPIO增强前及增强后24 h MRI扫描.扫描序列为SE T1WI、SE T2WI、GRE T2＊ WI.各组大鼠扫描结束后留取膝关节滑膜行病理检查.多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验.结果 模型组T1WI增强前后各时间点未见明显滑膜信号改变,T2WI增强前及增强后24、48 h滑膜信噪比分别为24.13±1.96、17.09±1.23、19.14±0.91,T2＊WI增强前及增强后24、48 h滑膜信噪比分别为22.28±0.92、11.40±0.53、17.18±0.63,T2WI及T2＊WI增强后24、48 h滑膜信噪比较增强前差异均有统计学意义（P＜0.05）;T2WI增强前后滑膜信噪比改变（△SNR）于增强后24、48、72 h分别为-29.1±2.4、-20.8±2.9、-6.2±2.9,T2 ＊WI增强前后滑膜△SNR于增强后24、48、72 h分别为-48.4±1.3、-22.9±0.8、-8.2±1.6,均于增强后24 h变化最明显（P＜0.05）,病理示增强后24 h膝关节滑膜内铁粒子沉积较多;T2WI模型对照组及来氟米特组增强前后滑膜△SNR分别为-32.6±0.6、-29.0±0.8,T2 ＊WI模型对照组及来氟米特组增强前后滑膜△SNR分别为-44.2±1.2、-40.1±1.9,来氟米特组增强前后滑膜△SNR较模型对照组弱（P＜0.01）,病理证实来氟米特组巨噬细胞及铁粒子沉积较模型对照组均减少.结论 USPIO增强磁共振对CIA大鼠关节滑膜巨噬细胞成像在SET2WI及GRE T2＊ WI序列表现为信号减低;USPIO增强后24 h MRI较48 h成像更佳;来氟米特干预后USPIO增强前后MRI滑膜信号改变减弱.关节滑膜巨噬细胞MRI有望成为临床RA诊断及药物疗效监测的新技�Objective To explore the optimal time and sequence for getting the best magnetic resonance （MR） imaging when MR image of synovium macrophages was used for the diagnosis of collageninduced arthritis （CIA） in a rat model,and whether it can be used to monitor the efficacy of drug treatment.Methods CIA was induced by subcutaneous injection of chicken type Ⅱ collagen and complete Freund＇s adjuvant （CFA）.Arthritis rats were randomly divided into the model group,the leflunomide group and the control group.Knees of the model group rats were imaged before and 24 h,48 h,72 h after USPIO intravenous administration （300 μmol Fe/kg） on day 28,29,30,31,respectively.From day 28,the leflunomide group was given a gauge of drug at a dose of 8 mg/kg.Then they were imaged before and 24 hours after USPIO administration on day 42,43 respectively.MR sequences included SE T1WI,SE T2WI,GRE T2 ＊ WI.After the completion of MR imaging,rats were sacrificed to obtain histopathologic samples of synovial membrane.LSD-t test and one-way analysis of variance （ANOVA） were used for statistical analysis.Results No distinct signal enhancements were observed on the 24 h,48 h,72 h post-contrast enhancement on T1WI.On T2WI,signal intensity ratio of synovium （SNR） pre-contrast and 24 h,48 h post-contrast were 24.13±1.96,17.09± 1.23,19.14±0.91,respectively.On T2 ＊ WI,SNR pre-contrast and 24 h,48 h post-contrast were 22.28±0.92,11.40±0.53,17.18±0.63,respectively.Distinct signal changes were observed on 24 h,48 h post-contrast on T2WI and T2 ＊ WI （P〈0.05）.The changes between SNR at 24 h,48 h,72 h post-contrast and pre-contrast were-29.09±2.42,-20.83±2.90,-6.2±2.9 respectively on T2WI,which were-48.4±1.3,-22.9±0.8,-8.2±1.6 respectively on T2 ＊ WI.Changes were more obvious at 24 h post-contrast than 48 h post-contrast on both T2WI and T2 ＊ WI （P〈0.05）.The quantitative analyses were coinci-dent with the visual differences in signal changes between pre-contrast and post-contrast images.Difference be

关 键 词：关节炎 实验性 磁共振成像 巨噬细胞 超微超顺磁氧化铁粒子 来氟米特 

分 类 号：R684.3[医药卫生—骨科学]