乙醇诱导人脐静脉内皮细胞凋亡及对ICAM-1蛋白表达影响的研究  

Effect of human umbilical vein endothelial cells apoptosis induced by ethanol and the expression of ICAM-1

作　　者：何胜虎[1,2] 周胜华[1] 张晶[2] 王雪飞[2] 王大新[2] 

机构地区：[1]中南大学湘雅二院 [2]江苏省苏北人民医院心内科

出　　处：《中国临床药理学与治疗学》2015年第3期266-268,308,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:研究乙醇体外诱导人脐静脉内皮细胞(HUVEC)凋亡,及对细胞间黏附分子(ICAM-1)蛋白表达的影响。方法:以不同浓度乙醇(50、100和200mmol/L)作用于体外培养的人脐静脉内皮细胞24h,四甲基偶氮唑盐(MTT)比色法测定各组细胞活力;流式细胞仪检测细胞凋亡率;酶联免疫吸附法检测ICAM-1蛋白表达。结果:乙醇能抑制内皮细胞活力,促进细胞凋亡,增加ICAM-1表达,并呈浓度依赖性。结论:乙醇能诱导内皮细胞损伤,其机制可能与促进细胞凋亡、影响ICAM-1蛋白表达有关。AIM：To study the apoptosis induced by ethanol on human umbilical vein endothelial cells（HUVEC）and the expression of ICAM-1 in vitro. METHODS： HUVEC were treated with different concentrations of ethanol（50,100 and 200mmol/L）for 24 hours,the activity of cells was determined by methyl thiazolyl tetrazolium（MTT）assay;the rate of apoptosis was detected by flow cytometry（FCM）assay;and the expression of ICAM-1by enzyme linked immunosorbent assay.RESULTS：Ethanol can inhibit the cells activity,promote the apoptosis,increase the expression of ICAM-1of HUVEC in dose-dependent manner.CONCLUSION：Ethanol can damage endothelial cells and its possible mechanism may be linked to promote the apoptosis and the expression of ICAM-1.

关 键 词：乙醇 人脐静脉内皮细胞 细胞间黏附分子 细胞凋亡 

分 类 号：R965.2[医药卫生—药理学]