通督调神针灸预处理对脑缺血再灌注大鼠相关微小RNA调控机制的研究  

Effect of Electroacupuncture and Moxibustion Pretreatment on Expression of Cerebral microRNAs and Aquaporin Protein-4 in Cerebral Infarction Rats

作　　者：郑仕平[1] 韩为[1] 储浩然[1] 王颖[1] 张玲[1] 朱玲玲[1] 张国庆[1] 崔倩倩[1] 

机构地区：[1]安徽中医药大学第二附属医院,合肥230061

出　　处：《针刺研究》2015年第2期99-103,共5页Acupuncture Research

基　　金：安徽省自然科学基金项目(No.1308085MH 174)

摘　　要：目的:观察通督调神针灸预处理对脑缺血再灌注大鼠相关微小RNA(microRNA,miRNAs)、水通道蛋白-4(Aquaporin-4,AQP 4)表达的影响,探讨通督调神针灸预处理脑保护的作用机制。方法 :Wistar大鼠随机选取10只作为空白组,其余120只分为电针预处理组、艾灸预处理组、阿司匹林预处理组及模型组各30只。采用Longa线栓法制备脑缺血再灌注模型。电针和艾灸预处理组取"百会""风府""大椎"穴。电针预处理组进行电针治疗,艾灸预处理组用清艾条悬灸治疗,阿司匹林预处理组用阿司匹林10mg/kg灌胃治疗,治疗7d后各组造模,并于再灌注24h后,运用实时荧光PCR和Western blot法分别检测皮质区相关miRNAs、AQP 4的表达。结果:与空白组相比,模型组miRNA 290、miRNA 494相对表达量均明显降低(P<0.01);与模型组相比,各预处理组miRNA 290、miRNA 494相对表达量均显著提高(P<0.01);与阿司匹林预处理组相比,电针预处理组和艾灸预处理组miRNA 290、miRNA 494相对表达量均显著提高(P<0.01);与艾灸预处理组相比,电针预处理组相对表达量显著提高(P<0.05)。与空白组相比,模型组AQP 4相对表达量显著提高(P<0.01);与模型组相比,各预处理组AQP 4相对表达量均显著降低(P<0.05,P<0.01);与阿司匹林预处理组相比,电针预处理组和艾灸预处理组AQP 4相对表达量均显著降低(P<0.01,P<0.05);与艾灸预处理组相比,电针预处理组相对表达量降低更明显(P<0.05)。结论:通督调神针灸预处理是预防脑梗死的有效方法,通督调神针灸预处理脑保护机制之一可能是通过提高miRNA 290、miRNA 494的表达,降低AQP 4相对表达量,诱导脑缺血耐受,减轻脑水肿实现的。Objective The present study aimed at observing the effect of moxibustion pretreatment on the expression of cerebral microRNAs and Aquaporin protein-4 （AQP 4） in rats with cerebral ischemia and reperfusion （CI/R）, so as to reveal its mechanism underlying improvement of cerebral infarction. Methods A total of 130 Wistar rats were randomly divided into blank control（n = 10）, CI/R model（n = 30）, electroacupuncture （EA, n = 30）, moxibustion（ n -- 30）, Aspirin groups （ n = 30）. Before modeling, EA （2 Hz/5 Hz, 1-2 mA） or moxibustion was applied to ＂Baihui＇（GV 20）, ＂Fengfu＇（GV 16） and ＂Dazhui＂ （GV 14） for 20 min, once daily for 7 days. The rats of the Asprin group were treated by intragastric administration of Aspirin （10 mg/kg, 1 mg/mL）, once daily for 7 days before modeling. The CI/R model was established by occlusion of the bilateral carotid arteries. The expression levels of cerebral miRNAs and AQP 4 were detected by real-time PCR and Western blot, respectively. Results Compared with the blank control group, the expression levels of cerebral miRNA 290 and miRNA 494 were significantly reduced, while that of AQP 4 was obviously up-regulated in the model group（ P^0.01 ）. After pretreatment with EA and moxibustion, the relative expression levels of miRNA 290 and miRNA 494 were significantlY higher in the EA, moxibustion and Aspirin pretreatment groups than in the model group （P〈0.01）, while cortical AQP 4 expression levels were significantly lower in the EA, moxibustion and Aspirin pretreatment groups than in the model group （P^0.01, P^0.05）. The effects of both EA and moxibustion groups were significantly superior to those of Aspirin pretreatrnent group in up-regulating expression of miRNA 290 and miRNA 494 and down-regulating expression ofAQP 4 （P〈0.01, P〈0.05）. In addition, the EA pretreatment was markedly superior to moxibustion pretreatment in the aforementioned effects （P〈0.05）. Cenclusion EA pretreatment of GV 14, GV 16 and GV

关 键 词：脑缺血再灌注 针刺预处理 艾灸预处理 大脑皮质微小 RNA 水通道蛋白-4 

分 类 号：R245.97[医药卫生—针灸推拿学]