针刺捻转补泻手法对应激性血压升高大鼠血浆乙酰胆碱及胸主动脉一氧化氮合酶、环鸟苷酸含量的影响  

Effect of Twirling-reinforcing-reducing Needling Manipulations on Contents of Serum Acetylcholine and Arterial NOS and cGMP in Stress-induced Hypertension Rats

作　　者：刘炜[1,2] 朱陵群[1] 陈思思 路树超 唐杰[5] 刘清国[2] 

机构地区：[1]北京中医药大学东直门医院教育部中医内科学重点实验室,北京100007 [2]北京中医药大学针灸推拿学院,北京100029 [3]九江市中医医院,九江332000 [4]北京市宣武中医医院,北京100050 [5]北京市昌平区中西医结合医院,北京102208

出　　处：《针刺研究》2015年第2期136-140,共5页Acupuncture Research

基　　金：国家自然科学基金(No.30772833/81072861)

摘　　要：目的:观察针刺捻转补泻手法对应激性血压升高大鼠的血压及乙酰胆碱(Ach)/一氧化氮(NO)/环鸟苷酸(cGMP)传导途径的影响,探讨捻转补泻手法调控血压的效应机制。方法:60只Wistar大鼠随机分为空白组和造模组,造模组采用电击加噪声刺激15d制成应激性血压升高模型。造模成功的动物随机分为模型组、针刺组、捻转补法组、捻转泻法组,每组12只。各治疗组动物每日分别施以双侧"太冲"穴针刺不做手法、捻转补法和泻法操作。7d后,用ELISA法检测各组大鼠血浆Ach、胸主动脉一氧化氮合酶(NOS)和cGMP的含量。在造模前、造模后、针刺后分别测量动物收缩压。结果:与空白组比较,造模后各组大鼠血压明显上升(P<0.01);针刺7d后,与模型组比较,各治疗组血压均有所降低(P<0.05,P<0.01),其中捻转泻法组较其它两个治疗组降低更明显(P<0.05)。与空白组比较,模型组血浆Ach、动脉NOS和cGMP含量均明显下降(P<0.01);与模型组比较,3个治疗组血浆Ach、动脉NOS含量均显著升高(P<0.01,P<0.05),捻转补法和泻法组的动脉cGMP含量也显著升高(P<0.01);3个治疗组中,捻转泻法组Ach含量升高更明显(P<0.01),捻转补法组NOS含量以及捻转补法和泻法组cGMP含量的升高较针刺组显著(P<0.05,P<0.01)。结论:针刺捻转泻手法可以有明显的降压作用,其作用机制可能是通过调整Ach/NO/cGMP实现的。Objective To observe the effect of twirling-reinforcing or reducing needling manipulations on plasma acetylcholine （Ach） content and expression of nitric oxide synthetase （NOS） and cyclic guanosine monophosphate （cGMP） in thoracic artery tissue in stress-induced hypertension rats. Methods A total of 60 male rats were randomly divided into blank control, model, acupuncture （no-needle-manipulation）, twirling-reinforcing needling and twirling-reducing needling groups （n = 12 in each group）. The stress hypertension model was established by giving the animals with noise and electric shock＇stimulation （paw）, twice a day for15 days. Acupuncture stimulation was applied to bilateral ＂Taichong＂ （LR 3） for 1 min, followed by retaining the needles for 20 min. The treatment was conducted once daily for 7 days. Systolic blood pressure of the rat＇s tail was detected with non-invasive method and plasma Ach, and NOS and cGMP contents in the thoracic artery tissue were measured using ELISA method. Results Compared with the control group, the systolic blood pressure waS significantly higher in the model group after 15 days＇ stress stimulation （P〈0.01）, while the contents of plasma Ach, arterial NOS and cGMP were markedly down-regulated （P〈0. 01）. Following 7 days＇ acupuncture interventions, the increased blood pressure was down-regulated in the no-needle-manipulation, twirling-reinforcing needling and twirling-reducing needling groups （ P〈0.05, P〈0.01 ） ; and the decreased Ach and NOS in the 3 treatment groups ,＇ and cGMP levels in the twirling-reinforcing and twirling-reducing needling groups were remarkably up-regulated （P〈0.01, P〈0. 05）. No significant change of arterial cGMP content was found in the no-needle-manipulation group （P〉0.05）. The effect of the twirling-reducing needling was superior to that of no-needle-manipulation and twirling-reinforcing needling in lowering blood pressure and raising plasma Ach content （P〈0.05, P〈0.01）. Conclusio

关 键 词：针刺 应激性血压升高 捻转补泻 血浆乙酰胆碱 动脉一氧化氮合酶及环鸟苷酸 

分 类 号：R245.311[医药卫生—针灸推拿学]