半边旗提取物5F对乳腺癌细胞内活性氧水平的影响  

Effects of 5F from Pteris semiinnata L. on Reactive Oxygen Species Levels in Breast Cancer Cells

作　　者：邱万寿[1] 刘威[2] 吴珏堃[1] 李玺[1] 龙梅珺[1] 

机构地区：[1]中山大学附属第三医院甲乳外科,广东广州510630 [2]暨南大学医学院第四附属医院,广州市红十字会医院乳腺科,广东广州510220

出　　处：《今日药学》2015年第3期163-166,共4页Pharmacy Today

基　　金：广东省女性肿瘤用药研究基金项目(2014D09)

摘　　要：目的观察中草药半边旗提取物5F(Ent-11a-hydroxy-15-oxo-kaur-16-en-19-oic-acid,5F)对乳腺癌细胞的抑制作用及其对乳腺癌细胞内活性氧(reactive oxygen species,ROS)水平的影响。方法不同浓度(0、5、10、20、40μg/m L)的5F分别处理3种不同乳腺癌细胞(MCF-7、MDA-MB-231以及SK-BR-3),用CCK-8法检测5F对不同乳腺癌细胞株增殖的影响;流式细胞仪检测5F处理后的各乳腺癌细胞株的凋亡率;检测不同浓度的5F处理后的乳腺癌细胞内ROS的水平。结果 5F可抑制MDA-MB-231、MCF-7、SK-BR-3三种乳腺癌细胞的增殖,其抑制效果与5F的浓度及作用时间呈显著正相关(P<0.05);经5F作用24 h后,MDA-MB-231细胞、MCF-7细胞及SK-BR-3细胞的凋亡率较阴性对照组明显增加(P<0.05);5F作用于乳腺癌细胞株,ROS相对含量均较未处理组降低,且随5F浓度升高ROS水平降低越明显(P<0.05)。结论 5F可抑制乳腺癌细胞的增殖,其抑制效果与5F浓度和作用时间相关;5F能降低细胞内ROS的生成水平,并呈现剂量依赖性。OBJECTIVE To explore the anti-cancer effects and effects on reactive oxygen species levels of ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid（ 5F） in human breast cancer cells. METHODS Human breast cancer cells（ MCF-7,MDA-MB-231,SKBR-3） were treated with 5F in different concentrations（ 0,5,10,20,40 μg / m L） for different periods respectively. Cell viability was detected by a cell counting kit-8. The appotosis rates of cells were examined by flow cytometer. To detect the level of ROS in breast cancer cells after treated with different concentrations of 5F. RESULTS 5F inhibited the proliferation of MDA-MB-231,MCF-7,SKBR-3 in a concentration- and time-dependent manner（ P 0. 05）. After treated by 5F for 24 h,the apoptosis rate of MDA-MB-231,MCF-7,SK-BR-3 were increased significantly when compared with the control group（ P 0. 05）. The ROS level of 5F in patients of the treated group were lower than that of the untreated groups,and in a concentration-dependent manner（ P 0. 05）. CONCLUSION5 F could inhibit the proliferation of breast cancer cells in a concentration- and time-dependent manner. 5F can reduce intracellular ROS levels in a concentration-dependent manner.

关 键 词：半边旗 5F 乳腺癌细胞 活性氧 

分 类 号：R95[医药卫生—药学]