HPLC法测定芪丹颗粒剂中黄芪甲苷的含量  

Content determination of astragaloside in qidan granules by HPLC

作　　者：关欣[1] 徐雅娟[2] 解生旭[2] 刘悦[2] 司云珊[2] 刘云雪[1] 张丁文[1] 

机构地区：[1]长春中医药大学,长春130117 [2]吉林省中医药科学院,长春130021

出　　处：《长春中医药大学学报》2015年第2期245-247,共3页Journal of Changchun University of Chinese Medicine

基　　金：科技部重大创制药物专项(2009ZX09103-447)

摘　　要：目的建立芪丹颗粒剂中黄芪甲苷的HPLC含量测定方法。方法通过HPLC法对芪丹颗粒剂中黄芪甲苷进行含量测定及方法学考察。结果经测定黄芪甲苷在芪丹颗粒剂中的含量为0.165 mg/g。标准品黄芪甲苷在0.28~3.36μg范围内呈良好的线性关系,精密度RSD=0.43%（n=5）;稳定性良好,RSD=0.31%;重现性RSD值为RSD=1.44%（n=5）。平均回收率为97.94%,RSD值为1.44%（n=5）。结论该方法简单,精密,专属性好,可为芪丹颗粒剂质量标准研究奠定基础。Objective To establish the method of content determination of astragaloside in qidan granules by HPLC.Methods Content determination and methodological study were conducted in astragaloside in qidan granules by HPLC method. Results It was determined that the content of astragaloside in qidan granules was 0. 165 mg / g; the content of was 0. 305 mg / g. Standard astragaloside within the range of 0. 28 ~ 3. 36 μg presented good linear relationship,its precision RSD = 0. 43%（ n = 5）; its stability was good,RSD = 0. 31%; its reproducibility RSD value was RSD= 1. 44%（ n = 5）; its average recovery rate was 97. 94% and its RSD value was 1. 44%（ n = 5）. Conclusion The method was simple and accurate with good specificity,and laid foundation for the quality standard research of qidan granules.

关 键 词：HPLC 芪丹颗粒剂 黄芪甲苷 含量测定 

分 类 号：R248.2[医药卫生—中医临床基础]