南海短尾大眼鲷的种群遗传结构分析  

Genetic structure of Priacanthus macracanthus population from the South China Sea

作　　者：熊丹[1,2] 李敏[1] 陈作志[1] 李永振 李玉芳[1,2] 黄梓荣[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,农业部南海渔业资源环境科学观测实验站,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306 [3]农业部南海区渔政局,广东广州510300

出　　处：《南方水产科学》2015年第2期27-34,共8页South China Fisheries Science

基　　金：农业部财政重大专项(NFZX2013);国家科技支撑计划项目(2013BAD13B06);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所资助项目)(2014TS23);农业部财政专项“南海北部近海渔业资源调查”(20141005)

摘　　要：在南海北部陆架和南海南沙西南陆架6个地理位点采集224尾短尾大眼鲷（Priacanthus macracanthus）样品,采用线粒体控制区（mt DNA D-loop区）序列分析了其种群遗传结构,探讨了6个采样位点之间短尾大眼鲷种群归属关系。所得224条同源序列D-loop片段（729 bp）中检测到101个突变位点和94个核苷酸多态位点,定义了172种单倍型;遗传多样性表现出高单倍型多样性（0.980 5-0.997 1）和低核苷酸多样性（0.048 2-0.060 9）的特点;系统发育分析、分子方差分析和成对的Fst分析显示南海各群体之间有较高的遗传同质性,遗传分化不显著,也没有出现明显的地理分支或聚簇;中性检测和不对称分布分析发现南海的短尾大眼鲷群体发生过种群扩张。结果表明,南海海域的6个短尾大眼鲷群体属于同一种群,该结果为今后中国与南海周边国家渔业资源共享和争取捕捞配额提供了依据。We investigated the genetic structure of red bigeye（ Priacanthus macracanthus） population in six sampling sites off the South China Sea including five coastal waters of Beibu Gulf,Sanya,Maoming,Zhuhai,Shantou and the Southwest Nansha. Sequences of the mitochondrial control region（ D-loop） were amplified by polymerase chain reaction. Fragments of 729 bp of the mitochondrial D-loop were sequenced from 224 individuals,and 101 mutated sites,94 nucleotide polymorphism sites as well as 172 haplotypes were identified. All the six groups were characterized with high haplotype diversity（ 0. 980 5 - 0. 997 1） and low nucleotide diversity（ 0. 048 2 - 0. 060 9）. Analyses of phylogeny,molecular variances and pairwise Fstshowed no significant genealogical clades or clusters of samples corresponding to sampling localities. Both neutrality tests and mismatch distribution analyses revealed a recent population expansion in P. macracanthus. Thus,the six P. macracanthus groups in the South China Sea belong to one population. The results provide references for the fishery resources sharing and quotas winning between China and the South China Sea surrounding countries.

关 键 词：种群判别 遗传结构 线粒体控制区 短尾大眼鲷 南海 

分 类 号：Q347[生物学—遗传学] S931.5[农业科学—渔业资源]