胶原-纤维蛋白胶膜复合脐带间充质干细胞修复小鼠全层皮肤创面的实验研究  

Umbilical cord mesenchymal stem cell combined with collagen-fibrin double layer membrane accelerates wound healing

作　　者：南文滨[1] 陈红丽[1] 刘瑞[2] 徐志浩[1] 原志庆[1] 

机构地区：[1]新乡医学院生命科学技术学院,453003 [2]新乡医学院三全学院,453003

出　　处：《中华损伤与修复杂志（电子版）》2015年第1期41-45,共5页Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)

基　　金：河南省教育厅自然科学研究计划(13B180214);河南省教育厅自然科学研究计划项目(2011B310006);新乡医学院科研培育基金资助(2014QN137)

摘　　要：目的制备胶原-纤维蛋白胶双层复合材料,评估其作为皮肤组织工程材料复合人脐带间充质干细胞(h UCMSC)修复小鼠全层皮肤创伤的效果。方法制备胶原-纤维蛋白双层材料,扫描电镜检测双层支架材料表面结构。24只小鼠建立全层皮肤创面模型后,随机分为3组:空白组用无菌纱布处理伤口、h UCMSC复合胶原组和h UCMSC复合胶原-纤维蛋白胶组,分别采用各组敷料处理创面。治疗后5、10、15 d,观察创面愈合情况,计算3组创面愈合率并进行对比。治疗后15 d,取小鼠创面新生皮肤组织苏木精-伊红染色,观察新生皮肤组织结构。结果扫描电镜结果显示:双层材料胶原层横断面呈现出高孔径结构并且互相连通,纤维蛋白胶层横断面显示紧密的三维网状结构;创面愈合结果显示:h UCMSC复合胶原-纤维蛋白胶组治疗5、10、15 d创面愈合率分别达到(62.62±6.27)%、(88.47±7.14)%、(96.14±4.56)%,优于空白组(t=17.03、16.22、15.69,P均<0.01),同时也优于h UCMSC复合胶原组(t=2.71、5.60、5.28,P均<0.05)。h UCMSC复合胶原-纤维蛋白胶组组织学检查新生皮肤组织结构良好。结论胶原-纤维蛋白胶双层支架具有较好的三维空间结构,复合h UCMSC后有利于促进皮肤创面愈合。Objective Preparation of collagen- fibrin double layer membrane. To study the effect of human umbilical cord mesenchymal stem cell （hUCMSC） combined with collagen-fibrin double layer membrane on wound healing in mice. Methods Preparation of collagen-fibrin double layer membrane. The surface stueture of the double loyer membrane was investigated by SEM. Twenty-four mouse were prepaird as full thickness skin wound model and randomly divided into 3 groups： control group was bound up their wounds by conventional method, hUCMSC combined with collagen membrane treated group and hUCMSC combined with collagen-fibrin double layer membrane treated group. Results The SEM results showed that collagen-layer exhibited a highly porous and interconnected structure. The wound healing rate in double layer membrane combined with hUCMSC treated group at 5,10,15 day was （62.62±6.27 ） % , （ 88.47±7.14）% ,（96.14±4.56）%, better than control group （t = 17. 03, 16. 22, 15.69, P 〈 0.01）, and hUCMSC combined with collagen membrane treated group （t = 2. 71, 5.60, 5.28, P 〈 0. 05 ）, differences were significant. Conclusions Collagen-fibrin double layer membrane showed a good three dimensional space structure, combined with hUCMSC could accelerate wound healing.

关 键 词：创伤和损伤 间质干细胞 脐血干细胞移植 纤维蛋白 胶原I型 组织工程 

分 类 号：R641[医药卫生—外科学]