HPV16L1基因甲基化在不同民族宫颈癌中的相关性分析  

Correlation analysis on HPV16L1 gene methylation in cervical cancer patients of different nationalities

作　　者：纪莎[1] 玛依努尔.尼牙孜 隋霜[2] 朱开春[2] 

机构地区：[1]石河子大学医学院,新疆石河子832000 [2]新疆维吾尔自治区人民医院妇科

出　　处：《中国妇幼保健》2015年第12期1920-1923,共4页Maternal and Child Health Care of China

基　　金：国家自然科学基金〔81360367〕

摘　　要：目的：探讨HPV16L1基因甲基化水平在维吾尔族和汉族女性宫颈癌之间的差异性;并探讨HPV16L1基因13个位点（5927、5963、6367、6389、6457、6581、6650、6731、6797、7034、7091、7136、7145）的甲基化率在同民族中的差异和在维吾尔族、汉族中的关联性,及其与病毒载量之间的相关性。方法：选取2012年9月-2014年10月在新疆维吾尔自治区人民医院妇科门诊及病房收集的宫颈标本108份,其中维吾尔族64份,汉族44份。采用焦磷酸测序的方法分别检测宫颈癌的HPV16L1 13个甲基化位点的基因甲基化率。结果：两民族宫颈癌标本13个甲基化位点甲基化率比较,差异无统计学意义（P〉0.05）。维吾尔族宫颈癌患者13个甲基化位点的甲基化之间的相关性：以位点5927为例,位点5927与剩余所有位点甲基化率之间的相关系数比较,差异有统计学意义（P〈0.05）。汉族宫颈癌患者13个甲基化位点的甲基化之间的相关性：以位点5927为例,位点5927与位点5963、6367、6389、6457、6650、6731甲基化率之间的相关系数比较,差异有统计学意义（P〈0.05）。HPV16L1基因13个位点的甲基化率在维吾尔族和汉族中,在6367、6389两个基因位点关联性密切。维吾尔族宫颈癌标本位点6797、7145的甲基化率与病毒载量之间的相关系数比较,差异有统计学意义（P〈0.05）。结论：HPV16L1基因甲基化在维吾尔族和汉族间无差异,但在单一民族内有差异,甲基化率与病毒载量有关。Objective： To explore the difference of HPV16L1 gene methylation between cervical cancer patients of Han nationality and Uygur nationality,the differences of methylation rates of 13 HPV16L1 gene sites（ 5927,5963,6367,6389,6457,6581,6650,6731,6797,7034,7091,7136,7145） among the cervical cancer patients of the same nationality,the correlations between cervical cancer patients of Han nationality and Uygur nationality,and their correlations with viral load. Methods： A total of 108 cervical specimens collected from outpatient department and wards in Xinjiang People＇ s Hospital from September 2012 to October 2014 were selected,64 patients of Uygur nationality and 44 patients of Han nationality were included. Pyrosequencing was used to detect methylation rates of 13 HPV16L1 gene sites.Results： There was no statistically significant difference in the methylation rates of 13 HPV16L1 gene sites between the two groups（ P〈 0. 05）. Among the cervical cancer patients of Uygur nationality,there were statistically significant differences in the methylation rates between 5927 site and the other sites,respectively（ P〈0. 05）. Among the cervical cancer patients of Han nationality,there were statistically significant differences in the methylation rates between 5927 site and 5963,6367,6389,6457,6650,6731 sites,respectively（ P〈0. 05）.Among all the patients,the methylation rate of 6367 site was closely correlated with the methylation rate of 6389 site. There were statistically significant differences in the methylation rate and viral load between 6797 site and 7145 site among the cervical cancer patients of Uygur nationality（ P0. 05）. Conclusion： There is no significant difference in HPV16L1 gene methylation between cervical cancer patients of Han nationality and Uygur nationality,but there are differences within a single nationality,methylation rate is correlated with viral load.

关 键 词：HPV16L1基因 甲基化 宫颈癌 维吾尔族 汉族 

分 类 号：R711.74[医药卫生—妇产科学]