益智胶囊中9种有效成分的UPLC-MS/MS法测定  

Quantitative Analysis of Nine Active Constituents in Yizhi Capsule by UPLC-MS/MS

作　　者：李雪娜[1] 欧阳玥 刘晓燕 李向军[1,2] 刘敏彦 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150000 [2]河北以岭医药研究院有限公司,河北石家庄050035 [3]河北省络病重点实验室,河北石家庄050035 [4]国家中医药管理局重点研究室(心脑血管络病),河北石家庄050035

出　　处：《中国医药工业杂志》2015年第4期381-384,共4页Chinese Journal of Pharmaceuticals

基　　金：国家重点基础研究发展计划(973计划)项目(2012CB518606)

摘　　要：建立了超高效液相色谱串联质谱法同时检测益智胶囊中的9种成分——原儿茶醛、绿原酸、紫丁香苷、隐绿原酸、松果菊苷、毛蕊花糖苷、迷迭香酸、丹酚酸B和丹酚酸A的含量。采用Waters Acquity UPLC BEH C18色谱柱,以乙腈∶0.1%甲酸为流动相,梯度洗脱,负离子模式检测。结果 9种有效成分在检测的范围内线性关系良好,方法回收率为97.7%~101.1%,RSD均小于3%。An UPLC-MS/MS method was established for the simultaneous determination of nine active compounds in Yizhi capsule, including protocatechuic aldehyde, chlorogenic acid, syringin, 4-dicaffeoylquinic acid, echinacoside, verbascoside, rosmarinic acid, salvianolic acid B and salvianolic acid A. A Waters Acquity UPLC BEH C18 column was used with the mobile phase of acetonitrile∶0.1% aqueous formic acid by gradient elution. The monitoring of all analytes was achieved under the negative ionization mode. All calibration curves showed good linearity within the test ranges, and the average recoveries were in the range of 97.7%- 101.1%, with RSDs less than 3%.

关 键 词：益智胶囊 超高效液相色谱串联质谱 测定 

分 类 号：R917[医药卫生—药物分析学] O657[医药卫生—药学]