蒲药灌肠液的质量控制  

Quality Control of Puyao Rectal Solution

作　　者：李燕[1] 赵利刚[2] 王春艳[1] 于桂兰[1] 张立新[1] 王洪民[1] 

机构地区：[1]唐山市妇幼保健院药剂科,河北唐山063000 [2]河北联合大学药学院,河北唐山063000

出　　处：《中国药房》2015年第12期1693-1695,共3页China Pharmacy

基　　金：河北省自然科学基金资助项目(No.H2013209098)

摘　　要：目的：建立蒲药灌肠液的质量控制方法。方法：采用薄层色谱法对乳香、黄芪甲苷和延胡索乙素进行定性鉴别,采用高效液相色谱法同时测定香蒲新苷和异鼠李素-3-O-新橙皮苷的含量。结果：薄层色谱均检出乳香、黄芪甲苷和延胡索乙素,斑点清晰,专属性强;含量测定中香蒲新苷和异鼠李素-3-O-新橙皮苷的线性范围分别为0.216 0~3.240μg（r=0.999 9,n=6）和0.208 8~3.132μg（r=0.999 8,n=6）,加样回收率分别为98.6%（RSD=1.3%,n=6）和98.4%（RSD=1.4%,n=6）。结论：该方法简便可行,结果准确可靠,可用于蒲药灌肠液的质量控制。OBJECTIVE：To establish a method for the quality control of Puyao rectal solution. METHODS：Thin layer chromatography（TLC）method was used to identify frankincense,astragaloside A and tetrahydropalmatine;HPLC was used to determinate the content of typhaneoside and isorhamnetin-3-O-neohesperidoside. RESULTS：Frankincense,astragaloside A and tetrahydropalmatine were detected by TLC with clear spot and strong specificity. The linear ranges of typhaneoside and isorhamnetin-3-O-neohesperidoside were respectively 0.216 0-3.240 μg（r=0.999 9,n=6）and 0.208 8-3.132 μg（r=0.999 8,n=6）,the average recoveries were respectively 98.6%（RSD=1.3%,n=6）and 98.4%（RSD=1.4%,n=6）. CONCLUSIONS：The method is simple,accurate,and can be used for the quality control of Puyao rectal solution.

关 键 词：蒲药灌肠液 香蒲新苷 异鼠李素-3-O-新橙皮苷 高效液相色谱法 质量控制 

分 类 号：R917[医药卫生—药物分析学]