TLCS法与HPLC法测定二至丸中齐墩果酸含量的方法比较  

Methods Comparison of the Content Determination of Oleanic Acid in Erzhi Pill by TLCS and HPLC

作　　者：孙小玲 何凡[2] 宿亚柳 

机构地区：[1]辽宁省药品检验检测院,沈阳110032 [2]辽宁中医药大学药学院,沈阳110847

出　　处：《中国药房》2015年第12期1696-1697,共2页China Pharmacy

摘　　要：目的：比较薄层扫描（TLCS）法与高效液相色谱（HPLC）法测定二至丸中齐墩果酸含量的方法。方法：分别采用TLCS法和HPLC法。TLCS法参照2010年版《中国药典》（一部）的方法,波长分别为530、680 mm。HPLC法采用色谱柱为ODS,流动相为乙腈-水（80∶20,V/V）,检测波长为220 nm,流速为1 ml/min,柱温为25℃。结果：HPLC法中,齐墩果酸的进样量在0.200 68-1.003 4μg范围内与峰面积呈良好线性关系;精密度、稳定性、重复性试验的RSD〈2%;平均加样回收率为100.7%,RSD为0.7%（n=6）。TLCS法测得二至丸中齐墩果酸的含量为8.78 mg/g,HPLC法测得其含量为9.82 mg/g,二者比较差异无统计学意义（P〉0.05）。结论：该研究所建立的HPLC方法操作快速、简便,结果稳定、可靠,更适用于二至丸中齐墩果酸的含量测定。OBJECTIVE：To compare the content determination method for oleanic acid in Erzhi pill by TLCS and HPLC.METHODS：TLCS and HPLC were conducted. Chinese Pharmacopoeia（first part,2010 edition）was used for TLCS. In HPLC,the wavelengths were respectively 530 and 680 mm;the column was ODS with the mobile phase of acetonitrile-water（80 ∶ 20,V/V）at the flow rate of 1 ml/min;the detection wavelength was 220 nm and the temperature was 25 ℃. RESULTS：In HPLC,there was a good linear relationship between the volume and the peak area in the range of 0.200 68-1.003 4 μg;the RSDs of precision,stability and repeatability was less than 2% and the average recovery was 100.7%（RSD=0.7%,n=6）. TLCS showed that the content of oleanic acid in Erzhi pill was 8.78 mg/g and HPLC showed that the content was 9.82 mg/g,with no significant difference（P〉0.05）. CONCLUSIONS：The established HPLC is stable,reliable,rapid,easy and feasible. It is more suitable for the content determination of oleanolic acid in Erzhi pill.

关 键 词：二至丸 齐墩果酸 薄层色谱 高效液相色谱法 

分 类 号：R972[医药卫生—药品]