HPLC法测定糖肾康颗粒中丹皮酚的含量  

Content Determination of Paeonol in Tangshenkang Granules by HPLC

作　　者：宋钦兰[1] 林海青[1] 

机构地区：[1]山东中医药大学附属医院,济南250011

出　　处：《中国药房》2015年第12期1700-1701,共2页China Pharmacy

摘　　要：目的：建立测定糖肾康颗粒中丹皮酚含量的方法。方法：采用高效液相色谱法。色谱柱为Kromasil C18柱,流动相为甲醇-水（43∶57,V/V）,流速为1.0 ml/min,检测波长为274 nm,柱温为25℃,进样量为10μl。结果：丹皮酚进样量在0.093 04~0.465 2μg（r=0.999 9）范围内与峰面积线性关系良好;精密度、重复性、稳定性试验的RSD≤1.56%;平均加样回收率为98.33%,RSD=0.81%（n=6）。结论：该方法简便、准确、可靠,可用于糖肾康颗粒中丹皮酚的含量测定。OBJECTIVE：To establish a method for the content determination of paeonol in Tangshenkang granules. METHODS：HPLC was conducted. The column was Kromasil C18 with the mobile phase of methanol-water（43 ∶ 57,V/V）at the flow rate of 1.0ml/min;the detection wavelength was 274 nm;the temperature was 25 ℃ and the volume was 10 μ l. RESULTS：There was a good linear relationship between the volume of paenol and the peak area in the range of 0.093 04-0.465 2 μ g（r=0.999 9）;the RSD of precision,repeatability and stability test was no more than 1.56% and the average recovery was 98.33%（RSD=0.81%,n=6）. CONCLUSIONS：The method is simple,accurate,replicable and can be used for the content determination of paeonol in Tangshenkang granules.

关 键 词：糖肾康颗粒 丹皮酚 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]