儿童肺炎支原体肺炎血清和肺泡灌洗液中T细胞亚群及细胞因子检测的意义  

The Clinical Significance of T-Cell Subset,Ceytokine Levels in Serum and BALF of Children with Mycoplasma Pneumoniae Pneumonia

作　　者：吴小磊[1] 张慧玉[1] 田玲[1] 庞保东[1] 付辉[1] 杨晓莹[1] 严慧[1] 

机构地区：[1]河北省唐山市妇幼保健院,河北唐山063000

出　　处：《儿科药学杂志》2015年第4期6-9,共4页Journal of Pediatric Pharmacy

摘　　要：目的:探讨T细胞亚群、白细胞介素-5(IL-5)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)在肺炎支原体肺炎(MPP)患儿血清和肺泡灌洗液中的水平及临床意义。方法:选取2012年12月至2014年5月在我院住院的肺炎患儿150例,包括难治性MPP(RMPP)患儿40例(RMPP组)、MPP伴喘息患儿40例(MPP1组)、MPP不伴喘息患儿40例(MPP2组)、非肺炎支原体肺炎且不伴喘息的普通肺炎患儿30例(对照组),应用流式细胞仪测定血清和肺泡灌洗液(BALF)中T细胞亚群水平,并采用双抗体夹心ELISA法测定IL-5、IL-6、IL-10、TNF-α水平,分析其临床意义。结果:RMPP组血清和BALF中CD3、CD4、CD4/CD8水平均低于MPP1组、MPP2组及对照组(P<0.05);RMPP组BALF中CD8水平低于MPP1组、MPP2组及对照组(P<0.05),血清CD8水平与MPP1组、MPP2组及对照组比较差异无统计学意义(P>0.05);MPP1组、MPP2组与对照组比较,血清和BALF中CD3、CD4、CD8、CD4/CD8水平差异无统计学意义(P>0.05)。RMPP组、MPP1组、MPP2组血清及BALF中IL-5、IL-6、IL-10、TNF-α水平均高于对照组(P<0.05),RMPP组血清及BALF中IL-6、IL-10、TNF-α高于MPP1组和MPP2组(P<0.05),MPP1组血清及BALF中IL-5高于RMPP组和MPP2组(P<0.05),MPP1组血清及BALF中IL-10低于MPP2组(P<0.05),MPP1组血清及BALF中IL-6、TNF-α高于MPP2组(P<0.05)。结论:IL-5、IL-6、IL-10、TNF-α参与了MPP的发病过程,且在MP感染诱发哮喘的发生、发展中起了重要作用。Objective： To explore the levels and clinical significance of T-cell subset,IL-5,IL-6,IL-10,TNF-α in serum and BALF of children with mycoplasma pneumoniae pneumonia. Methods： One hundred and fifty children with mycoplasma pneumoniae pneumonia were divided refractory MPP 40 cases（ RMPP group）,MPP with wheezing or without 40 each（ MPP1 or MPP2 group）,and30 children with pneumonia from non-MP infection（ control group） were enrolled. The levels of T-cell subset in serum and BALF were detected by flow cytometer,and the levels of IL-5,IL-6,IL-10,TNF-α were detected by ELISA. Results： The CD3,CD4,CD4 / CD8 levels of serum and BALF in RMPP group were significantly lower than those in MPP1 group,MPP2 group and control group（ P〈0. 05）,the CD8 levels of BALF in RMPP group were significantly lower than those in MPP1 group,MPP2 group and control group（ P〈0. 05）,There were no significant differences in serum level of CD8 between RMPP group,MPP1 group,MPP2 group and control group（ P〉0. 05）.There were no significant differences in CD3,CD4,CD8,CD4 / CD8 levels of serum and BALF between MPP1 group,MPP2 group and control group（ P〉0. 05）. The IL-5,IL-6,IL-10,TNF-α levels in serum and BALF in RMPP group,MPP1 group,MPP2 group were significantly higher than those in the control group（ P 〈 0. 05）,serum and BALF levels of IL-6,IL-10,TNF-α in RMPP group were significantly higher than those in MPP1 group and MPP2 group（ P 〈 0. 05）,serum and BALF levels of IL-5 in MPP1 group were significantly higher than those in RMPP group and MPP2 group（ P 〈 0. 05）,serum and BALF levels of IL-10 in MPP1 group were significantly lower than those in MPP2 group（ P 〈 0. 05）,serum and BALF levels of IL-6,TNF-α in MPP1 group were significantly higher than those in MPP2 group（ P〈0. 05）. Conclusion： IL-5,IL-6,IL-10 and TNF-α may participate in the pathogenesis of MPP,and plays an important role in occurrence and development of wheezing.

关 键 词：肺炎支原体 肺炎 肺泡灌洗液 T细胞亚群 白细胞介素 肿瘤坏死因子-α 

分 类 号：R725.6[医药卫生—儿科]