苍柏湿毒清及其拆方对解脲支原体感染小鼠IL-12影响  

The effect of Cangbaishiduqing and separated prescription on IL-12 level of ureaplasma urealyticum infeced mice

作　　者：聂晶[1] 李楠[2] 李元文[2] 蒋静[1] 蔡玲玲[2] 陈雪燕[1] 张历元 

机构地区：[1]北京中医药大学第二临床医学院,北京100029 [2]北京中医药大学东方医院皮肤科,北京100078

出　　处：《中国性科学》2015年第3期68-71,共4页Chinese Journal of Human Sexuality

基　　金：国家自然科学基金(81072811)

摘　　要：目的:观察苍柏湿毒清及其拆方对解脲支原体感染BALB/c小鼠阴道黏膜IL-12的影响。方法:选用解脲支原体血清4型经阴道建立BALB/c mice小鼠支原体感染模型。予苍柏湿毒清组、活血利湿组、清热解毒组、健脾利湿组中药灌胃7d。采用ELASA法检测小鼠阴道黏膜IL-12水平。结果:模型组IL-12值高于空白组(P<0.05);苍柏湿毒清组、健脾利湿组IL-12值低于模型组(P<0.05),与空白组差异不大(P>0.05)。结论:感染解脲支原体后,小鼠阴道黏膜IL-12水平升高,导致局部红肿、糜烂,炎症明显。苍柏湿毒清及健脾利湿类药物可将阴道黏膜IL-12水平恢复至正常,减轻小鼠阴道炎症。Objectives： To explore the effect of Cangbaishiduqing and separated prescription on IL- 12 level of Ureaplasma urealyticum infeced mice. Method： Mycoplasma infection models of 4 ureaplasma urealyticum serotypes of BALB / c mice were selected. The Cangbaishiduqing group,activating blood dampness group,Qingrejiedu group and Jianpilishi group were given TCM intragastric administration for 7 days. ELASA method was used to detect vaginal mucosa IL- 12 levels of mice. Results： The IL- 12 value of model group was higher than that of blank group（ P〈 0. 05）. The IL- 12 values of Cangbaishiduqing group and Jianpilishi group,which had no difference from the blank group,was lower than the model group＇s（ P〈 0. 05）. Conclusions： After being infected with mycoplasma urealytium,the IL- 12 level of mice on vaginal mucosa increases,which leads to the swelling and erosion with obvious inflammation. The Cangbaishiduqing and Jianpilishi medicine can reduce the IL- 12 levels on vaginal mucosa to normal level and reduce the vagina inflammation of mice.

关 键 词：解脲支原体 苍柏湿毒清 拆方 白介素12 

分 类 号：R759[医药卫生—皮肤病学与性病学]