甲基苯丙胺滥用人员尿液代谢组学初探  

METABONOMICS ON URINE OF METHAMPHETAMINE ABUSERS

作　　者：王玫[1] 吴明建[2] 张大明[1] 

机构地区：[1]北京市公安局法医检验鉴定中心,北京100192 [2]北京市公安局刑侦总队,北京100006

出　　处：《中国药物依赖性杂志》2015年第2期107-111,共5页Chinese Journal of Drug Dependence

摘　　要：目的:应用代谢组学的研究方法,对已经证实的甲基苯丙胺(MA)滥用人员尿液进行分析,筛选潜在的相关生物标志物。方法:运用高效液相色谱联合离子阱-飞行时间质谱(LC/MS-IT-TOF)对20名甲基苯丙胺滥用者与20名正常人的尿液进行检测。结果:多变量统计分析结果表明,甲基苯丙胺滥用者和正常人聚类明显,在尿液中共发现20种可能的生物标志物。结论:甲基苯丙胺滥用人员和正常人员在尿液代谢水平上具有明显差异,这为发现甲基苯丙胺滥用判定的潜在标志物提供了依据。Object： To select possible related markers among the urine of methamphetamine （MA） abusers by application of metabolomics research methods. Meths：Urine of 20 MA abusers and 20 healthy controls were investigated by Liquid Chromatography Coupled with Ion Trap - Time of Flight Mass Spectrometry（LC/MS- IT- TOF）. Results： Multivariate statistical analysis was performed to discriminate metabolite profiles of 20 MA addicts and 20 healthy controls. Twenty potential biomarkers were found in urine. Conclusion：The significant differences existed between these two groups on urine metabolic levels. These metabolites provided the basis which help to find potential biomarkers to determinate MA abusers.

关 键 词：甲基苯丙胺 代谢组学 液相色谱联合离子阱-飞行时间质谱 

分 类 号：R446.12[医药卫生—诊断学] R971.7[医药卫生—临床医学]