去氢木香内酯对实验性大鼠肝损伤的保护作用  

The Protective Effect of Dehydrocostus Lactone on Liver Injury in Laboratory Rats

作　　者：孙文利[1,2] 张晓旭[1,2] 黄志英[2,3] 陈畅[2] 康琛[2] 高健[2] 段飞鹏[4] 田朋朋[1,2] 陈坤[4] 李德凤[2] 刘玉欣[1] 李韶菁[2] 

机构地区：[1]河北大学药学院,保定071002 [2]中国中医科学院中药研究所,北京100700 [3]中国药科大学药学院,南京211198 [4]北京中医药大学,北京100102

出　　处：《世界中医药》2015年第3期399-402,共4页World Chinese Medicine

基　　金：重大新药创制国家科技重大专项(编号:2012ZX09103201-055);国家自然科学基金项目(编号:81274133;81303261);中国中医科学院自主选题项目(编号:ZZ2014005;ZZ2014060)

摘　　要：目的:研究去氢木香内酯对大鼠肝损伤模型的保护作用。方法:以氨基半乳糖、四氯化碳建立大鼠肝损伤模型,分别设正常对照组、模型组、阳性药对照组(0.2 mg/kg)、去氢木香内酯高、中、低剂量组(45 mg/kg,15 mg/kg,5 mg/kg),测定各组大鼠血清中丙氨酸氨基转移酶(Alanine Aminotransferase,ALT)、天门冬酸氨基转移酶(Aspartate Aminotransferase,AST)活性及肝组织中丙二醛(Malondialdehyde,MDA)含量。另以HE、Masson染色,对各组大鼠肝脏作病理检查。结果:与模型组相比,去氢木香内酯可显著降低大鼠血清ALT、AST的活性及MDA的含量(P<0.05),减轻大鼠肝脏坏死性病理改变。结论:去氢木香内酯对大鼠肝脏损伤有较好的保护作用。Objective： To investigate the protective effect of Dehydrocostus lactone on liver injury in experimental rats. Methods：Rats were injected with galactosamine and carbon tetrachloride to induce liver injury. The model rats were randomly divided into normal group,vehicle control,positive control group（ 0. 2 mg·kg- 1）,high,middle,and low Dehydrocostus lactone dose groups（ 45,15 and 5 mg·kg）. The changes of activity of ALT,AST in serum and MDA content in liver tissue were measured. The pathologic change of liver tissue was examined under light microscope by HE and Masson staining. Results： Compared with the vehicle group,Dehydrocostus lactone remarkably decreased（ P 0. 05） the activities of ALT,AST in serum and MDA content in liver tissue. It was observed that Dehydrocostus lactone could significantly alleviate the necrotic pathologic change of liver tissue.Conclusion： Dehydrocostus lactone has protective effects on liver injury in labortary rats.

关 键 词：去氢木香内酯 肝损伤 氨基半乳糖 四氯化碳 

分 类 号：R285.5[医药卫生—中药学]