人巨细胞病毒包膜糖蛋白B线性B细胞抗原表位的预测  

B cell line epitopes prediction of human cytomegalovirus glycoprotein B

作　　者：闫晶晶[1,2] 吕茂民[2] 赵雄[2] 尹惠琼[2] 章金刚[1,2] 

机构地区：[1]广西医科大学,南宁530021 [2]军事医学科学院野战输血研究所,国家生物医学分析中心病毒安全检测实验室,北京100850

出　　处：《军事医学》2015年第3期184-188,共5页Military Medical Sciences

基　　金：国家863计划资助项目(2012AA021902)

摘　　要：目的通过生物信息学方法分析人巨细胞病毒(human cytomegalovirus,HCMV)包膜糖蛋白B(glycoprotein B,g B)的结构及理化特性,预测g B的线性B细胞抗原表位。方法基于HCMV g B的序列,利用两种在线B细胞表位预测程序及DNAstar软件对g B进行预测;利用SWISS-MODEL服务器同源构建g B三级结构模型,进行进一步验证。结果与结论综合多项分析,预测得到HCMVg B的多个线性B细胞表位,为后续验证其优势中和表位,建立富含高效价中和抗体的原料血浆的筛选方法奠定了基础。Objective To predict the B cell line epitopes of human cytomegalovirus glycoprotein （gB）by analyzing its structure and physicochemical properties using bioinformatics approaches. Methods Based on the sequence of the HCMV gB,the probable B cell epitopes are predicted using two online prediction programs and DNAstar software. Meanwhile, the tertiary structure of gB was constructed by homologous modeling with the assistance of SWISS-MODEL server to rule out impossible B cell epitopes. Results and Conclusion The B cell line epitopes of gB are predicted, which provides a theoretical basis for further verification of gB immunodominant epitopes and screening the source plasma with high HCMV IgG titer.

关 键 词：人巨细胞病毒 糖蛋白B B细胞表位 

分 类 号：R373.11[医药卫生—病原生物学]