血小板保存专用塑料血袋的储存性能评价  

Evaluation of storage performance of special plastic blood bags for apheresis platelets

作　　者：王捷熙[1] 周倩[2] 刘敏霞 王艳[1] 柴丽娜[1] 卓海龙[3] 易晓阳[1] 周建伟[1] 王建卫 韩颖[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850 [2]北京市红十字血液中心,北京100088 [3]解放军307医院输血科,北京100071 [4]山东威高集团医用高分子制品股份有限公司,山东威海264209

出　　处：《军事医学》2015年第3期206-210,共5页Military Medical Sciences

基　　金：国家自然科学基金青年资助项目(81100333)

摘　　要：目的以美国Gambro BCT公司生产的Trima set血小板保存袋为对照,评价山东威高集团医用高分子制品股份有限公司生产的血小板保存袋对单采血小板的储存性能。方法将1 U单采血小板平分成两份,分别加入实验血袋和对照血袋中,于血小板恒温振荡保存箱中保存。分别于保存0、3、5、7 d取样,比较分析两组的血小板含量和血小板平均体积、p H值、体外聚集活性、氧分压和二氧化碳分压、葡萄糖、乳酸和乳酸脱氢酶浓度、低渗休克反应、血小板活化和凋亡、血小板形态结构及细菌培养试验。结果两种保存袋保存的血小板在保存期内,相同保存时间实验组与对照组之间各项检测结果差异无统计学意义(P>0.05);无菌试验均为阴性。结论国产血小板专用塑料血袋与美国Trima set血小板保存袋对该实验使用的15份单采血小板具有相似的储存性能。Objective To evaluate the storage performance of storage bags for apheresis platelets produced by Shandong Weigao Group Medical Polymer Co. , Ltd （experimental bags） with Trima set platelet storage bags produced by the U. S. Gambro BCT as the control. Method One unit of apheresis platelets was divided into two equal parts, added to control blood bags and experimental blood bags respectively. All samples were stored at （22±2）℃ with consecutive oscillation. The platelets＇ count, mean volume, aggregate activity （ADP, THR）, pH, glucose, lactate concentration, lactate dehydro- genase concentration, hypotonic shock reaction, expression of CD62P and phosphatidyl serine on surface of cell membrane were detected at 0,3,5 and 7 d respectively. Results There was no significant difference in platelet quality after five days of storage between the experimental group and the control group （t-test, P 〉 0.05）. Conclusion Two types of platelet storage blood bags have similar storage performance for apheresis platelets.

关 键 词：血小板 血袋 储存性能 

分 类 号：R457[医药卫生—治疗学]