蓝莓茎段组培快繁技术研究  

Research on Rapid Propagation of Stem Segment of the Blueberry

作　　者：沙玉芬 王建萍[1] 李公存[1] 顾亮[1] 苏佳明[1] 

机构地区：[1]山东省烟台市农业科学研究院,山东烟台265500

出　　处：《安徽农业科学》2015年第10期27-28,共2页Journal of Anhui Agricultural Sciences

基　　金：烟台市科技发展计划项目(2014HZ105)

摘　　要：[目的]研究蓝莓茎段组培快繁技术。[方法]以北高丛蓝莓品种‘杜克’为试材,春季一年生新梢茎段为材料进行组培快繁。[结果]进瓶最佳灭菌时间为8-10 min,成活率达75%-88%;最佳增殖培养基为改良WPM＋ZT 1.0 mg/L,增殖系数为12.8,生长健壮,平均株高4.8 cm;最佳生根培养基为1/2WPM＋IBA1.0 mg/L,生根率达100%,平均根长2.1 cm;最佳移栽基质是草炭土＋蛭石,成活率达92.6%。[结论]该研究对蓝莓快速繁育和推广种植具有一定的意义。[Objective] The aim was to study rapid propagation of the stem segment of blueberry. [ Method ] Using stem segment of newshoots as material in autuam, the tissue culture of Northernhighbush Blueberry ＇ Bluecrop＇ was studied. [ Result ] Results indicated that the best sterilization time was 8 - 10 min, the survival rate reached 75 % - 88 %. The best proliferation medium was improved WPM ＋ ZT1.0 mg/L, the prolif- eration rate reached 12.8, and the average height was 4.8 cm. The best rooting medium was 1/2WPM ＋ IBA1.0 mg/L, the rooting rate reached 100% ,the average length was 2.1 cm. The best transplanting medium was peatsoil mixed with vermiculite ,the survival rate reached 92.6%. [Conclusion] The study had certain significance for fast breeding and planting of the blueberry.

关 键 词：蓝莓 组织培养 茎段 

分 类 号：S663.2[农业科学—果树学]