Analysis of Genetic Relationships in Prunus domestica L. in Xinjiang Using ISSR Markers  

基于ISSR分子标记的新疆欧洲李亲缘关系分析（英文）

作　　者：孙琪[1] 廖康[1] 耿文娟[1] 刘娟[1] 曼苏尔.那斯尔 刘欢[1] 贾杨[1] 曹倩[1] 

机构地区：[1]新疆农业大学特色果树研究中心,新疆乌鲁木齐830052

出　　处：《Agricultural Science & Technology》2015年第3期447-453,503,共8页农业科学与技术（英文版）

基　　金：野生欧洲李亲缘关系研究;国家自然科学基金项目(31160387);新疆维吾尔自治区果树重点学科基金~~

摘　　要：[Objective] This paper aimed to explore the genetic relationships among different types of Prunus domestica L. in Xinjiang. [Method] The inter-simple se- quence repeat （ISSR） markers were applied to analyze the genetic diversity and relationships among 30 individual plants from 5 types of P. domestica germpiasm resources in Xinjiang. [Result] A total of 317 bands were amplified by 16 selected IS- SR primers. Among the amplified bands, there were 246 polymorphic bands, accounting for 77.60% of the total. The Nei＇s gene diversity index （H） of individuals was 0.266 6. The average Shannon＇s information index （I） of individuals was 0.399 1. And most of the genetic similarity coefficients ranged from 0.555 2 to 0.996 8. It is indicated that the P. domestica germplasm resources have a certain genetic diversity in Xinjiang. The cluster analysis showed that, at the genetic similarity coefficient of 0.719, the 30 individuals were divided into 3 major groups, including a group of introduced European plum varieties, a group of Tacheng Smoked Plum and Tacheng Binzi and a group of Jiashi Smoked Plum and Wild European Plum. While at the genetic similarity coefficient of 0.949, Jiashi Smoked Plum and Wild European Plum were divided into two small groups. [Conclusion] There are very close genetic relationships between Jiashi Smoked Plum and Wild European Plum, and among the introduced European plum varieties, Tacheng Smoked Plum and Tacheng Binzi.[目的]探究不同新疆欧洲李种质类型间的亲缘关系。[方法]运用ISSR分子标记对5个类型共30个单株的新疆欧洲李种质资源进行遗传多样性及亲缘关系分析。[结果]筛选获得的16条引物共扩增出317条带,其中有多态性条带246条,占扩增总数的77.60%。供试单株的Nei’s遗传多样性指数（H）为0.266 6,平均Shannon信息多样性指数（I）为0.399 1,遗传相似系数多在0.555 2~0.996 8之间,表明新疆欧洲李资源具有一定的遗传多样性。聚类分析显示,在遗传相似系数0.719处30株供试欧洲李被划分为3个大组,国外引进欧洲李品种一组、塔城酸梅和塔城槟子一组、伽师酸梅和野生欧洲李一组,而在相似系数0.949处,伽师酸梅和野生欧洲李又分为两个小组。[结论]伽师酸梅与野生欧洲李的亲缘关系很近,国外引进欧洲李品种与塔城酸梅及塔城槟子的亲缘关系较近。

关 键 词：XINJIANG Prunus domestica L. ISSR Genetic relationship 

分 类 号：S662.3[农业科学—果树学]