沈阳市2013年HIV抗体初筛阳性标本的复检及确证结果分析  

Analysis of rescreening and confirmation for 934 anti- HIV antibody positive samples in primary screening of Shenyang in 2013

作　　者：刘建敏[1] 董雪[1] 李欣[1] 刘蕴博[1] 王琪[1] 张国斌[1] 

机构地区：[1]沈阳市疾病预防控制中心,辽宁沈阳110031

出　　处：《中国卫生检验杂志》2015年第6期844-845,851,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的分析沈阳市934份HIV抗体筛查阳性标本的复检和确证实验结果,为沈阳市艾滋病防治工作提供依据。方法对初筛阳性的血清,用ELISA法和明胶颗粒凝集法(PA)复检,任一复检实验阳性的血清再用免疫印迹法(WB)进行确证实验,并对确证实验结果进行分析。结果 934份初筛阳性血清经过复检有847份呈阳性反应,经WB法确证660份为HIV-1抗体阳性,不确定98份,阴性89份。ELISA法和明胶颗粒凝集法与WB法的阳性符合率分别为80.9%和78.9%。结论采用ELISA法和明胶颗粒凝集法进行复检,可有效排除初筛实验室的假阳性标本,提高确证的阳性符合率,降低确证检测成本,但是2种方法均存在一定的假阳性。Objective To analyze rescreening and confirmation for 934 anti- HIV antibody positive samples in primary screening in Shenyang,so as to provide basis for prevention and control of AIDS. Methods Anti- HIV antibody positive in primary screening test samples were rescreened with both enzyme- linked immuno sorbent assay（ ELISA） and particle agglutination（ PA）. Then the positive samples in either rescreening test were confirmed with western blot（ WB）. Results In 934 positive samples from primary screening test,847 samples were positive in rescreening test. 660 cases were confirmed by WB test,while98 cases were uncertain and 89 cases were negative. In contrast to WB,positive coincidence rates of ELISA and PA were80. 9% and 78. 9% respectively. Conclusion Rescreening tests with ELISA and PA can increase the confirmed positive coincidence rate,and also cut the cost in the confirmation. However,there is still some false positive rate in either rescreening test.

关 键 词：人类免疫缺陷病毒 酶联免疫吸附测定法 明胶颗粒凝集法 免疫印迹法 

分 类 号：R446.61[医药卫生—诊断学]