紫外分光光度法测定不同产地黄芪总黄酮的含量  

Determination of Flavonoid in Astraga Lus Grown in Different Areas by UV Spectrophotometry

作　　者：程研[1] 闵会 何厚洪 胡江宁 吴健 王小东 王如伟[1,4] 

机构地区：[1]浙江中医药大学,杭州310053 [2]浙江康恩贝药品研究开发有限公司,杭州310052 [3]大兴安岭地区食品药品检验检测所,黑龙江鹤岗165000 [4]浙江康恩贝制药股份有限公司(浙江省中药制药技术重点实验室),杭州310052

出　　处：《江西中医药大学学报》2015年第1期58-60,共3页Journal of Jiangxi University of Chinese Medicine

基　　金：浙江省企业研究院项目(2010E90011)

摘　　要：目的：建立黄芪中总黄酮的含量测定方法,比较不同产地黄芪药材总黄酮的含量。方法：以毛蕊异黄酮苷为对照品,采用紫外分光光度法,测定波长为260 nm,对不同产地黄芪药材总黄酮的含量进行测定。结果：毛蕊异黄酮苷在4-20μg/m L呈现良好的线性关系,r=0.9999,平均回收率为99.96%,RSD%为1.53%;不同产地黄芪药材,总黄酮含量在0.234%-0.383%范围内。结论：本实验建立的方法适用于测定黄芪药材中总黄酮的含量;不同产地黄芪中总黄酮含量以毛蕊异黄酮苷计由高到低依次为大兴安岭、甘肃、山西、内蒙古。Objective：To establish a new method for determining the contents of flavonoid in AstragaLus among different areas. Meth- ods：Flavonoid in AstragaLus was determined by UV spectrophotometry at the wavelength 260 nm, with calycosin-7-glucoside as index. And this method can be used for assaying of flavonoid in AstragaLus from different habitats. Results：The linear range of calibration curve was 4-20 μg/mL, r=0.9999. The average recovery rate was 99.96% with the relative standard deviation 1.53%. The contents of flavonoid in AstragaLus were 0.234%-0.383%. Conclusion：This method can be used for evaluation of quality of flavonoid in AstragaLus. The contents in flavonoid can be ranked as follows： Daxinganling〉Gansu〉Shanxi〉Neimenggu.

关 键 词：紫外分光光度法 毛蕊异黄酮苷 总黄酮 含量测定 

分 类 号：R284.1[医药卫生—中药学]