疏血通注射液预防大鼠血管吻合口血栓形成的实验研究  

An experimental study of Shuxuetong( 疏血通) injection for prevention of vascular anastomotic stoma thrombosis in rats

作　　者：赵祚塨[1,2] 王孝辉[1,2] 姚俊娜[1,2] 郭绍勇[1,2] 陈利国[1,2] 段卫峰[1,2] 穆世民[1,2] 杜志军[1,2] 蔡利涛 

机构地区：[1]河南省洛阳正骨医院 [2]河南省骨科医院,河南洛阳471002

出　　处：《中医正骨》2015年第3期12-16,共5页The Journal of Traditional Chinese Orthopedics and Traumatology

摘　　要：目的:观察疏血通注射液预防大鼠血管吻合口血栓形成的疗效。方法:将120只Wistar大鼠随机分为对照组、疏血通组及肝素钠组,每组40只。对照组、疏血通组及肝素钠组大鼠分别以生理盐水(10 m L·kg-1)、疏血通注射液(2 g·kg-1)及肝素钠注射液(600 U·kg-1)进行腹腔注射。药物注射6 h后将所有大鼠左侧颈总动脉切断,并即刻对其进行端端吻合。分别于造模结束后30 min及1、3、7 d时从各组随机选取10只大鼠,切取包含吻合口远近端各0.5 cm的颈总动脉,在电子显微镜下观察并计算吻合口及缝线表面覆盖的纤维蛋白、血小板及内皮细胞的百分比。结果:1纤维蛋白覆盖率。造模后不同时间,3组大鼠血管吻合口纤维蛋白覆盖率比较,组间差异均有统计学意义(F=133.802,P=0.000;F=18.741,P=0.000;F=212.943,P=0.000;F=341.174,P=0.000)。造模后30 min时对照组纤维蛋白覆盖率低于疏血通组和肝素钠组(P=0.000,P=0.000);造模后1、3、7 d时对照组纤维蛋白覆盖率均高于疏血通组和肝素钠组(P=0.000,P=0.000;P=0.000,P=0.000;P=0.000,P=0.000);造模后3 d时疏血通组纤维蛋白覆盖率高于肝素钠组(P=0.000);其余各时点疏血通组和肝素钠组比较,组间差异均无统计学意义。2血小板覆盖率。造模后不同时间,3组大鼠血管吻合口血小板覆盖率比较,组间差异均有统计学意义(F=352.002,P=0.000;F=84.734,P=0.000;F=368.903,P=0.000;F=14.413,P=0.000)。造模后30 min、1 d、3 d、7 d时对照组血小板覆盖率均高于疏血通组和肝素钠组(P=0.000,P=0.000;P=0.000,P=0.000;P=0.000,P=0.000;P=0.000,P=0.000);造模结束后30 min时疏血通组血小板覆盖率高于肝素钠组(P=0.027);其余各时点疏血通组和肝素钠组比较,组间差异均无统计学意义。3内皮细胞覆盖率。除造模后30 min时外,其余各时点3组大鼠血管吻合口内皮细胞覆盖率比较,组间差异均有统计学意义(F=0.000,P=1.000;F=835.502,P=0.000;F=363.874,P=0.000;F=Objective：To observe the preventative effect of Shuxuetong（疏血通）injection on vascular anastomotic stoma thrombosis in rats.Methods：One hundred and twenty Wistar rats were randomly divided into control group,Shuxuetong group and heparin group,40 cases in each group.The Wistar rats in the 3 groups were intraperitoneal injected with normal saline（1 0 mL/kg）,Shuxuetong injection（2 g/kg） and heparin injection（600 U/kg）respectively.The left common carotid arteries（LCCA）of all rats were cut off at 6 hours after injection,and end-to-end anastomosis were performed instantly.Ten rats were randomly selected from each group at 30 minutes,1 ,3 and 7 days after the modeling respectively,and the common carotid arteries near the anastomotic stoma were fetched for observing.The fraction of coverage of fi-brin,blood platelets and endothelial cells at anastomotic stoma and suture surface were observed and calculated by using electron micro-scope.Results：There was statistical difference in coverage fraction of fibrin in vascular anastomotic stoma between the 3 groups after the modeling（F=1 33.802,P=0.000;F=1 8.741 ,P=0.000;F=21 2.943,P=0.000;F=341 .1 74,P=0.000）.The coverage fraction of fi-brin of control group was lower than that of Shuxuetong group and heparin group at 30 minutes after the modeling（P=0.000,P=0.000）. The coverage fraction of fibrin of control group was higher than that of Shuxuetong group and heparin group at 1 ,3 and 7 days after the modeling（P=0.000,P=0.000;P=0.000,P=0.000;P=0.000,P=0.000）.The coverage fraction of fibrin of Shuxuetong group was higher than that of heparin group at 3 days after the modeling（P=0.000）.There was no statistical difference in coverage fraction of fibrin between Shuxuetong group and heparin group at the rest timepoints.There was statistical difference in coverage fraction of blood platelets in vascular anastomotic stoma between the 3 groups after the modeling（F=352.002,P=0.000;F=84.734,P=0.000;F=368.903,P=0.000;F=1 4.41 3,P=0.000）.The c

关 键 词：血栓形成 疏血通 肝素 显微外科手术 血小板 纤维蛋白 内皮细胞 动物实验 

分 类 号：R285.5[医药卫生—中药学]