全自动凝聚胺微板法和微柱凝胶法检测红细胞抗体的对比  

Comparison of automatic polybrene microplate method and gel microcolumn method in detection of red blood cell antibodies

作　　者：许婷婷[1] 何鸣镝[1] 张丽洁[1] 江梦天[1] 任明[1] 沈钢[1] 

机构地区：[1]武汉血液中心,武汉430030

出　　处：《临床血液学杂志》2015年第2期224-225,共2页Journal of Clinical Hematology

基　　金：武汉市卫计委临床医学科研项目(No:WX13D08)

摘　　要：目的:比较全自动凝聚胺微板法和微柱凝胶法检测红细胞抗体的优劣,为实验室选择试验方法提供依据。方法:用2种方法对64例阳性抗体和64例阴性标本进行平行检测。结果:凝聚胺微板法检测出62例,2例抗-Lea漏检;微柱凝胶法检测出49例,13例IgM抗体及2例抗-E抗体漏检。结论:全自动凝聚胺微板法对抗体的检出率高。Objective：To compare the automatic polybrene microplate method and gel microcolumn method in detection of red blood cell antibodies. Method.. We parallelly detected 64 positive samples and 64 negative samples with two methods. Result：Sixty-two positive samples were found and 2 samples of anti-Le failed to he found by Polybrene microplate method. Forty-nine positive samples were found, 13 samples of IgM antibody and 2 samples of anti-E failed to be found by gel microcolumn method. Conclusion： Automatic Polybrene microplate method has higher detection rate of antibodies.

关 键 词：凝聚胺微板法 微柱凝胶法 不规则抗体 

分 类 号：R446.11[医药卫生—诊断学]