黑逍遥散对阿尔茨海默病模型大鼠行为学及血清肿瘤坏死因子α及白细胞介素-6和-1β的影响  

Effects of HeiXiaoYao Powder on the Behavior and the TNF-α, IL-6 and IL-1β Contents of Alzheimer Disease Rat Models

作　　者：杨长生[1] 吴红彦[1] 李海龙[1] 容倩倩 

机构地区：[1]甘肃中医学院,甘肃兰州730000

出　　处：《西部中医药》2015年第2期10-12,共3页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号81060284)

摘　　要：目的:观察黑逍遥散对β-淀粉样蛋白(Aβ25-35)所致阿尔茨海默病(Al zhei mer di s eas e,AD)模型大鼠血清肿瘤坏死因子(TNF-α)、白细胞介素(I L-6、I L-1β)表达的影响,探讨黑逍遥散防治AD的作用机制。方法:海马区注射Aβ25-35建立AD大鼠模型,用黑逍遥散、石杉碱甲进行对抗治疗,第28天取血清,通过酶联免疫吸附法(ELI SA)测定AD大鼠血清TNF-α、I L-6、I L-1β含量。结果:模型组大鼠血清中TNF-α、I L-6、I L-1β含量显著升高,各治疗组大鼠血清TNF-α、I L-6、I L-1β含量均有所降低,黑逍遥散高剂量组大鼠血清TNF-α、I L-6、I L-1β含量显著降低。结论:有效控制AD患者体内炎性因子表达是黑逍遥散的重要作用机制。Objective：To observe the effect of HeiXiaoYao powder on the rats with Alzheimer disease （AD） induced by Aβ 25-35 in the expression of tumor necrosis factor （TNF-α）, interleukin-1β （IL-1β） and interleukin-6 （IL-6） in blood serum and to study the functional mechanism of HeiXiaoYao powder in the prevention and treatment of AD. Methods： After the establishment of the AD rat models by injecting Aβ 25-35 in the hippocampus, the models were treated by HeiXiaoYao powder and huperzine A. On the 28th day, the contents of TNF-α, IL-6 and IL-1β in the serum of the models were exam ined by enzyme-linked immunosorbent assay （ELISA）. Results：The TNF-α, IL-6 and IL-1βcontents of the control group were increased hugely, while in the treatment group the con-tents of TNF-α, IL-6 and IL-1βwere all decreased and the HeiXiaoYao powder group of high dose were significantly decreased. Conclusion：The HeiXiaoYao powder can effectively control the expression of the inflammatory factor for AD patients, which is one of important mechanisms of HeiXiaoYao powder.

关 键 词：阿尔茨海默病 黑逍遥散 炎性因子 

分 类 号：R285.6[医药卫生—中药学]