用于工业杀菌剂评估的高通量筛选方法的建立  

High-throughput Screening Methods for Selecting Industrial Bactericide

作　　者：杨平 衣雪竹 

机构地区：[1]大连百傲化学股份有限公司,辽宁大连116038

出　　处：《广州化工》2015年第6期135-136,147,共3页GuangZhou Chemical Industry

摘　　要：为建立一种工业杀菌剂高通量筛选方法，采用96孔板培养法，以大肠杆菌作为待测菌，利用显色剂显色反应对杀菌剂的抑菌能力进行评价。结果显示，采用1～4 d培养菌液，稀释至10％或菌液吸光度值A＝0.038，显色剂TTC（2，3，5－氯化三苯基四氮唑）加入量20 mg／kg，显色18～24 h可得到与试管法一致的结果。该方法操作简捷，微量省力，结果易观察，成本低，可用于工业杀菌剂的大量筛选和应用评估。To establish an high throughput screening methods for industrial bactericide assessment, E.coli was using as tested bacterium, the bactericide antibacterial activity was studied based on color reaction which was determined by 96-well microtiter plates incubation method.The method was feasible, using 1~4 d culture broth which diluted to 10%or broth absorbance A=0.038, added TTC （2,3,5-TriphenyltetrazoliuM Chloride） 20 mg/kg and then cultivatd 18~24 h, can get the same result with the vitro methods.This method is simple to operate, trace and effort, easy to observe, low cost, can be used for a large number of screening and application assessment of industrial bactericides.

关 键 词：杀菌剂评估 高通量筛选 显色剂 

分 类 号：Q93-332[生物学—微生物学]