河北省鼠疫耶尔森菌多位点串联重复序列分析  

MLVA analysis on Yersinia pestis isolated from plague foci in Hebei province, China

作　　者：王海峰[1] 杨顺林[1] 周松[1] 史献明[1] 胡乐乐[1] 刘合智[1] 梁莹[2] 

机构地区：[1]河北省鼠疫防治所检验科,河北张家口075000 [2]中国疾病预防控制中心传染病预防控制所

出　　处：《中国媒介生物学及控制杂志》2015年第2期141-144,共4页Chinese Journal of Vector Biology and Control

基　　金：卫生部行业基金(201202021);河北省2014年度医学科学研究重点课题计划(ZL20140119)~~

摘　　要：目的对分离自河北省长爪沙鼠鼠疫疫源地的116株鼠疫菌株进行基因分析研究。方法根据鼠疫基因组比对,选择15对引物对测试菌株进行PCR扩增,并对扩增结果进行分析。结果 15对引物对116株实验菌株均得到相应的扩增结果,同一引物测试菌株扩增结果目标条带长度均一致,串联重复序列的重复数相同。不同引物扩增目标条带长度不同,串联重复序列的重复数也不同,如引物M52对所有菌株扩增产物长度均为153 bp,串联重复序列的重复数均为3,而引物M59的扩增产物长度为250 bp,重复数均为7。结论多位点串联重复序列分析(MLVA)证实河北省鼠疫自然疫源地的鼠疫菌基因稳定,鼠疫菌MLVA数据库将对未来的鼠疫菌变异和溯源提供技术支持。Objective To characterize the genosome of 116 Yersinia pestis strains isolated from plague foci in Hebei province in China. Methods All the strains were detected by polymerase chain reaction （PCR） with 15 pairs variable number tandem repeat （VNTR） provided by China CDC, then the length of results were analyzed. Results The numbers and lengths of the repeat sequences from all strains are same with the single primer, and different with the different primers, such as the length of all stains with the prime M52 is 153 bp, and the number of the repeat sequences is 3, then with the prime M59, the length and the number is 250 bp and 3 respectively. Conclusion Multiple loci VNTR analysis （MLVA） genetic typing is reliable, and it is stable genetic mark of Y. pestis from Hebei province. To build the database of the plague with MLVA is useful for the investigation of the Dlague variation and source.

关 键 词：鼠疫菌 多位点串联重复序列分析 基因分型 

分 类 号：R254.8[医药卫生—中医内科学]