秦皇岛口岸首例输入性登革热病例的分子溯源  

Molecular origins of the first imported dengue fever case at frontier port in Qinhuangdao

作　　者：杨鹏飞[1] 燕清丽[1] 张丽萍[2] 聂维忠[3] 甄维[2] 马雪征[2] 白红岩[2] 刘纯成[1] 时玉军 陈跃[1] 孙肖红[2] 胡孔新[2] 

机构地区：[1]淮安市疾病预防控制中心,江苏淮安223001 [2]中国检验检疫科学研究院 [3]秦皇岛出入境检验检疫局

出　　处：《中国媒介生物学及控制杂志》2015年第2期155-158,共4页Chinese Journal of Vector Biology and Control

基　　金：国家科技重大专项课题(2013ZX10004-101-006;2013ZX10004805-008)~~

摘　　要：目的对2012年秦皇岛口岸的首例登革热输入性病例进行分子溯源,确定病毒的基因型别及感染来源。方法应用ELISA方法和实时荧光RT-PCR方法对患者进行初筛,利用RT-PCR法扩增病毒的NS1基因并测序,根据获得的序列进行同源性分析、遗传距离计算及系统进化树的构建。结果从患者2份血清(QHD-01-BS1和QHD-01-BS2)中检测到Ig M抗体阳性,从QHD-01-BS1及尿液(QHD-01-US)中检测到登革热2型病毒的NS1基因。同源性分析发现QHD-01-BS1(US)与COM基因型中的印度株GWL39 INDI-01和GWL18 INDI-01同源性最高,为97.3%;QHD-01-BS1(US)的遗传距离与COM基因型遗传距离最小,为0.04;进化分析显示QHD-01-BS1(US)与GWL39 INDI-01和GWL18 INDI-01亲缘关系最近。结论分子溯源证实患者感染的登革热病毒与来源于印度的登革热2型病毒亲缘关系最近,指示其传染源极有可能在印度。Objective To trace the virus from an imported viral hemorrhagic fever case in Qinhuangdao in 2012, molecular characteristics of the pathogen were studied. Methods The serum collected from the suspected case was detected for IgM antibody by ELISA, and viral nucleic acid of serum and urine samples were detected by real-time RT-PCR. Furthermore, NS1 segment was amplified by RT- PCR and sequenced. The genetic and phylogenetic analysis based on NS1 was computed. Results IgM anti-DENV was found in the serum QHD-01-BS1, BS2, and the NS1 gene sequences of serotype 2 of Dengue virus （DEN2） were recovered from the serum QHD-01-BS1 and the urine QHD-01-US respectively. The comparison showed the strains QHD-01-BS1 and QHD-01-US were very closely related to GWL39 INDI-01, GWL18 INDI-01 the COM genotype） isolated from India, with 97.3% sequence identity and 0.04 genetic distance. The further phylogenetic tree indicated the strains QHD-01-BS1 and QHD-01-US were classified as a lineage with GWL39 INDI-01, GWL18 INDI-01, belonged to genotype COM. Conclusion These results suggest the imported viral hemorrhagic fever case was infected by DENV2 from India and most likely infected in India.

关 键 词：输入病例 登革热病毒 分子溯源 基因分型 

分 类 号：R373.33[医药卫生—病原生物学]