苦参碱对炎症状态下肾小管上皮细胞B7-H1mRNA及蛋白表达的影响  

Effect of matrine on the expression of B7-H1 in tubular epithelial cells

作　　者：吴瑗[1] 

机构地区：[1]广东医学院微生物与免疫学教研室,广东湛江524023

出　　处：《山东医药》2015年第2期5-7,共3页Shandong Medical Journal

基　　金：广东省中医药管理局科研基金资助项目(ZY0904);广东医学院科研基金面上项目(XK0801)

摘　　要：目的探讨苦参碱对炎症状态下肾小管上皮细胞B7-H1 mRNA及蛋白表达的影响。方法取人肾小管上皮细胞(HK-2)用于实验。1苦参碱浓度对B7-H1的影响:设对照组、模型组及苦参碱低、中、高剂量组,对照组仅加含5%胎牛血清的DMEM培养液,模型组及苦参碱低、中、高浓度组加入终浓度为100 g/L的IFN-γ,苦参素低、中、高浓度组再分别加入0.5、0.8、1.0 g/L的苦参碱;培养24 h,采用RT-PCR法检测HK-2细胞中的B7-H1 mRNA,流式细胞术检测B7-H1蛋白。2苦参碱干预时间对B7-H1的影响:设对照组、模型组及苦参碱不同培养时间组(12、16、24 h),对照组仅加含5%胎牛血清的DMEM培养液培养24 h,模型组加终浓度为100 g/L的IFN-γ培养24h,苦参碱12 h、16 h、24 h组加入终浓度为100 g/L的IFN-γ和0.8 g/L的苦参碱,分别培养12、16、24 h,采用RTPCR法检测HK-2细胞中的B7-H1 mRNA。结果模型组B7-H1 mRNA及其蛋白表达量高于对照组,苦参碱低、中、高浓度组低于模型组(P均﹤0.05);苦参碱12 h、16 h、24 h组B7-H1 mRNA及其蛋白表达低于模型组(P均﹤0.05)。苦参碱抑制HK-2细胞B7-H1 mRNA表达作用呈剂量、时间依赖性,对B7-H1蛋白的抑制作用也呈剂量依赖性(P均﹤0.05)。结论 IFN-γ诱导的炎症状态下肾小管上皮细胞B7-H1 mRNA、蛋白表达增高,苦参碱可下调B7-H1 mRNA、蛋白表达。Objective To explore the effect of matrine on expression of B7-H1 in tubular epithelial cells. Methods （~）The effect on different dose of matrine： human HK-2 cells were cultured under five culture conditions to observe and divided into 4 groups, which was control group（5% fetal calf serum ＋ DMEM for 24 h）, model group （ IFN-100 gilL for 24 h）, matrine group （IFN-100 g/L ＋ 0.8 g/L matrine for 12 h, 16 h and 24 h） . The expression of B7-H1 mRNA was evaluated by using real time RT-PCR. The surface expression of B7-H1 on HK-2 was analyzed by using flow cytometry. （~）The effect on different time of matrine： cell were divided into control group（5% fetal calf serum ＋ DMEM for 24 h） , model group （ IFN-100 g/L for 24 h） , matrine group （ IFN- 100 g/L ＋ 0.8 g/L matrine for 12 h, 16 h and 24 h）. The expression of B7-H1 mRNA was evaluated by using real time RT-PCR. Results The expression of B7-H1 on HK-2 ceils was decreased in normal condition. IFN- can significantly increased the expression of BT-H1. Matrine decreased the B7-H1 expression of HK-2 cells in a dose and time dependent way at mRNA levels and in a dose dependent way at protein levels （ P 〈 0.05 ）. Conclusion BT-H1 mRNA and protein increased under the condition of inflammation induced by IFN-γ. Matrine could down regulate the expression of BT-H1 mRNA and protein.

关 键 词：苦参碱 肾小管上皮细胞 B7-H1基因 协同刺激分子 

分 类 号：R692[医药卫生—泌尿科学]