基于微流控芯片电泳化学发光检测人单个红细胞中血红蛋白的含量  

Determination of Hemoglobin in Single Human Red Blood Cell by Microchip Electrophoresis with Chemiluminescence Detection

作　　者：李向堂[1] 张礼春[1] 赵书林[1] 

机构地区：[1]广西师范大学化学与药学学院,广西桂林541004

出　　处：《分析测试学报》2015年第3期308-313,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21327007)

摘　　要：运用自行设计组装的微流控芯片电泳化学发光检测装置和单细胞分析专用玻璃微流控芯片,建立了一种测定人单个血红细胞中血红蛋白（Hb）含量的新方法.该方法采用双T型的窄进样通道,宽反应通道及适中分离通道的玻璃微流控芯片,集成单个细胞的进样、固定、溶胞、分离和检测等操作于一块微流控芯片上.以pH 10.5的硼砂缓冲液为电泳介质,选用鲁米诺-过氧化氢化学发光体系,对人单个红细胞中血红蛋白的含量进行测定.血红蛋白的质量在2.0～90 pg范围内,与化学发光强度（峰高）呈良好的线性关系,检出限（S/N=3）为0.8 pg.通过对19个血红细胞进行检测,得到人单个血红细胞中血红蛋白的含量在14 ～ 68 pg范围内,该结果与无氰HGB测量法测得的总体细胞血红蛋白的平均值（34.5 pg）基本一致.In this paper, an application of microchip electrophoresis with chemiluminescence detec- tion（ MCE - CL） in single human red blood cell analysis was described. Human red blood cells were assayed to determine the intraeellular content of hemoglobin（Hb）. The injection of individual cells, in - line lysed, and MCE separation were integrated onto a simple cross mierofluidic chip. CL detec- tion was based on the catalytic effects of hemoglobin on the luminol - hydrogen peroxide reaction. The MCE - CL detection had a linear calibration curve over the range of 2.0 - 90 pg injected with a corre- lation coefficient of 0. 998 9 and a detection limit（ S/N = 3 ） of 0.8 pg. The content of Hb in individ- ual hmnan red blood cell was found in the range of 14 -68 pg（n = 19）. Compared to other methods, the MCE - CL method is simple and sensitive, and could be an attractive alternative for single cell analysis.

关 键 词：微流控芯片电泳 化学发光检测 单细胞分析 人血红细胞 血红蛋白 

分 类 号：O657.1[理学—分析化学] O629.7[理学—化学]