细胞剪切耐受性检测装置的设计、模拟及验证  

Design,simulation and validation of cells' shear stress tolerance detection device

作　　者：王晗[1] 夏建业[1] 张大鹤[1] 易小萍[1] 庄英萍[1] 

机构地区：[1]华东理工大学生物工程研究中心,上海200237

出　　处：《化学工程》2015年第3期5-8,32,共5页Chemical Engineering(China)

摘　　要：针对动物细胞培养过程中流场作用力对其的制约作用,文中借助计算流体力学的方法设计了一种新型的微流道装置。在该装置内可以产生分布均一而恒定的流场剪切力,并且大小与装置内液体的流速密切相关;同时本次研究采用了微尺度的粒子图像测速技术(PIV)对实验结果进行了验证。结果表明数值模拟得到的流场结构与PIV拍摄得到的流场非常接近,具有很好的吻合性,建模及计算结果是准确可信的。A new type of micro-fluid device was designed to study the effect of hydrodynamic force on animal cells culture employing the computational fluid mechanics, where the uniform and constant flow-field shear stress occurred, and the values of the force had a close relationship with the rate of liquid flow. The simulation result was verified by PIV technology for the complex one. The result shows that the flow field got from simulation is very similar to the result obtained by PIV and has a good consistency, thus the models and calculation results are accurate.

关 键 词：流体动力学 细胞黏附力 能量耗散率 微载体培养 

分 类 号：Q27[生物学—细胞生物学]