苗药黑骨藤部分理化指标及杠柳毒苷含量的测定  

Physicochemical Index and Determination of Miao Medicine Periploca forrestii

作　　者：刘育辰[1] 刘刚[1] 张树芬[1] 周涛[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《贵州农业科学》2015年第2期130-132,136,共4页Guizhou Agricultural Sciences

基　　金：贵州省中药现代化科技产业研究开发专项项目"基于功效组分地理变异的苗药黑骨藤的道地性研究"[黔科合ZY字(2013)3003];贵州省科学技术基金项目"苗药黑骨藤抗类风湿性关节炎药效物质基础的谱效关系学研究"[黔科合J字(2012)2069];贵阳中医学院博士基金项目"基于强心作用的黑骨藤质量标准研究";贵阳中医学院"大学生创新性实验"项目"苗药黑骨藤抗类风湿性关节炎有效部位指纹图谱研究"

摘　　要：为进一步完善苗药黑骨藤的质量标准评价体系,按照《中国药典》方法对10个不同产地黑骨藤药材中水浸出物、醇浸出物和水分含量进行测定,采用HPLC法测定药材中杠柳毒苷的含量,并采用薄层色谱(TLC)法对其进行定性鉴别。结果表明:该药材的薄层鉴别方法具有专属性,10批样品水溶性浸出物平均为8.97%,醇溶性浸出物含量平均为10.05%,水分含量平均为8.60%,杠柳毒苷含量平均为0.33mg/g。建立的方法准确、灵敏、简便,可用于苗药黑骨藤中杠柳毒苷的定性鉴别与含量测定。To further perfect the quality standard of miao medicine P .forrestii,water soluble extraction,ethanol soluble extraction and moisture of medicinal material from 10 producing areas were determined according to Chinese Pharmacopeia .P .forrestii was qualitatively determined by TLC using periplocin as control,the content of periplocin was determined by HPLC.Results：TLC identification were specified, the average contents of water soluble extraction, ethanol soluble extraction, water content,periplocin were 8.97%,10.05%,8.60%,0.33mg/g,respectively.The estab lished method, which is precise,sensitive and simgle,is suitable for qualitative identification and content determination of periplocin in Miao medicine P .forrestii.

关 键 词：黑骨藤 理化指标 杠柳毒苷 薄层色谱 高效液相色谱 含量测定 

分 类 号：S567.239[农业科学—中草药栽培]