爱玉种质资源遗传多样性的ISSR分析  

ISSR analysis on genetic diversity of ficus pumila var.awkeotsang germplasm resources

作　　者：史辉[1,2] 吴泽江[1] 阮少江[1,2] 陈勇[1,2] 

机构地区：[1]宁德师范学院生物系,福建宁德352100 [2]闽东特色生物资源福建省高校工程研究中心,福建宁德352100

出　　处：《宁德师范学院学报（自然科学版）》2015年第1期34-38,共5页Journal of Ningde Normal University(Natural Science)

基　　金：宁德师范学院"服务海西建设"项目(2010H309);福建省自然科学基金项目(2006JA0399);福建省大学生创新创业训练计划项目(201310398019)

摘　　要：采用ISSR分子标记方法对24份爱玉品种的遗传多样性进行分析。从100条ISSR引物中筛选出17条扩增清晰、重复性高、多态性好的引物,选取其中4条多态性强的引物进行聚类分析。结果表明：4条引物共检测到43个多态性位点,多态性百分率为89。6％。这4条引物可以将24份爱玉种质之间的遗传差异完全区分。利用聚类分析,可以将24份爱玉种质聚分为4大类,24份爱玉种质的遗传相似系数变化范围在0。69-0。92之间。The genetic diversity among 24 ficus pumila var.awkeotsang cultivars was analyzed by ISSR. ISSR genetic clustering analysis was then carried out on 4 primers generated from 17 polymorphic primers. The results showed that 43 polymorphic loci were detected from 4 ISSR primers. The percentage of polymorphic loci was 89.6%. The 24 germplasm resources can be completely separated by the 4 ISSR primers. 24 germplasm resources can be divided into 4 groups by cluster analysis. The genetic similarity of 24 germplasm resources ranges from 0.69-0.92.

关 键 词：爱玉 遗传多样性 亲缘关系 

分 类 号：S667[农业科学—果树学]