山东省枣疯病植原体的鉴定及分子变异分析  

Molecular identification and sequence polymorphism of phytoplasma associated with jujube witches' broom in Shandong Province

作　　者：陈妮[1,2] 刘永光[3] 仇平平[1] 刘文浩[1] 苏文敏[1] 竺晓平[1] 

机构地区：[1]山东农业大学植物保护学院植物病理系,泰安271018 [2]福山区农业局,福山265500 [3]潍坊科技学院,潍坊262700

出　　处：《植物病理学报》2015年第2期113-120,共8页Acta Phytopathologica Sinica

基　　金：山东省自然科学基金资助项目(ZR2012CM032)

摘　　要：本研究对山东省11个地区的枣疯病样品进行了鉴定和分子变异分析。以样品总DNA为模板,经扩增和序列测定,分别得到16SrRNA(1 432bp)、核糖体蛋白基因rp(1 196bp)、转运蛋白基因secA(836bp)和secY(1 421bp)的序列,secA基因序列是首次从枣疯病植原体中扩增获得。对获得的序列与NCBI数据库中相关植原体序列进行聚类和核苷酸变异分析,结果显示山东省枣疯病植原体属于16SrⅤ-B、rpⅤ-C、secYⅤ-C亚组,相对于16SrRNA基因,rp,secA和secY变异更大,非同义突变更多,更利于对国内不同来源的枣疯病植原体的精细系统进化分析。Jujube witches＇ broom phytoplasma （JWB） collected from Shandong Province was identified and the sequence polymorphism of some typical genes was then analyzed. Four different types of universal primer pairs of phytoplasma were used to amplify 1.4 kb fragment of 16S rRNA, 1.2 kb fragment of rp gene, 0.8 kb fragment of secA gene, 1.4 kb fragment of secY gene, and the secA gene was first amplified from jujube witches＇ broom phytoplama. The PCR-amplified products were sequenced and compared with the related sequences of phytoplasmas in NCBI. The results of phylogenetic analysis showed that jujube witches＇ broom phytoplasmas collected in Shandong belong to subgroup 16Sr V-B、 rp V-C、 secY V-C. Analysis of sequence polymorphism revealed rp, secA and secY contain more non-synonymous mutations and are more variable than 16S rRNA gene, indicating their suitable use for finer differentiation of diverse strains of the jujube witches＇ broom phytoplasmas from China.

关 键 词：枣疯病 植原体 分子鉴定 序列多态性 

分 类 号：S432.4[农业科学—植物病理学]