侵染扶桑和棉花的木尔坦棉花曲叶病毒侵染性克隆构建及致病性测定  

Construction and pathogenicity evaluation of infectious clones of Cotton leaf curl Multan virus infecting Hibiscus rosasinensis and Gossypium hirsutum

作　　者：顾周杭 胡高洁[1] 谢艳[1] 周雪平[1] 

机构地区：[1]浙江大学农业与生物技术学院生物技术所,杭州310058

出　　处：《植物病理学报》2015年第2期198-204,共7页Acta Phytopathologica Sinica

基　　金：公益性(农业)行业科研专项(201003065);国家自然科学基金资助项目(31171814)

摘　　要：从广东广州扶桑和广西南宁棉花中分离得到木尔坦棉花曲叶病毒(Cotton leaf curl Multan virus,CLCuMV)的2个分离物GD37和GX01,序列分析表明两分离物的基因组全序列同源性为99.4%,卫星分子序列同源性为99.2%。构建了GD37和GX01的侵染性克隆,农杆菌接种表明CLCuMV GD37能侵染本氏烟、心叶烟、三生烟、普通烟,GD37和GD37β共同接种产生叶片下卷、植株矮化等症状,但不能侵染棉花、番茄、矮牵牛;而分离自棉花的CLCuMV GX01能够侵染本氏烟,GX01和GX01β共同接种在本氏烟上产生叶片下卷皱缩等症状,但不能侵染棉花。Southern-blot结果表明在GD37单独侵染或与GD37β共同侵染的植株中均能检测到相应DNA分子的积累。In this study, two isolates of Cotton leaf curl Multan virus （CLCuMV）, GD37 and GX01 were obtained from Hibiscus rosasinensis in Guangdong province and Gossypium hirsutum in Guangxi province, respectively. The complete nucleotide sequence identity between CLCuMV GD37 and CLCuMV GX01 is 99.4%, and the homology between their associated betasatellites（GD37β and GX01β）is 99.2%. The infectious clones GD37 and GX01 were constructed and their pathogenicities were then tested by agroinoculation. It was showed that CLCuMV GD37 could infect Nicotiana benthamiana, N. glutinosa, N. tabacum Samsun and N. tabacum producing downward leaf curl symptoms, but could not infect cotton, tomato and petunia. Co-inoculation of CLCuMV GX01 with its betasatellite GX01β, which were isolated from infected cotton, could induce severe downward leaf curl symptoms in N. benthamiana, but could not infect cotton. Southern blot analysis showed CLCuMV DNA could be detected in both GD37- and GD37 ＆ GD3713-inoculated plants.

关 键 词：木尔坦棉花曲叶病毒 侵染性克隆 致病性 

分 类 号：S432.44[农业科学—植物病理学]